ArticleScientific reports2020
Spatiotemporal dynamics and quantitative analysis of phytoplasmas in insect vectors.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 42 citations in OpenAlex.
- Phytoplasma-plant interactions: effector-mediated host reprogramming, hormonal crosstalk, metabolic alterations and plant-mediated vector manipulation.World journal of microbiology & biotechnology · 2026Review
- Can the leafhopper microbiome unlock new strategies for its control?Journal of economic entomology · 2026Review
- Tracking the early spatio-temporal dynamics of phytoplasma multiplication within its leafhopper vector.Microbiology (Reading, England) · 2026Article
- First Report ofMicroorganisms · 2026Article
- On-Site Universal Phytoplasma Detection by 23S rRNA-Gene-Targeted Loop-Mediated Isothermal Amplification (LAMP).Methods in molecular biology (Clifton, N.J.) · 2026Article
- Effector innovation in genome-reduced phytoplasmas and other host-dependent mollicutes.PLoS genetics · 2025Article
- A phytoplasma effector suppresses insect melanization immune response to promote pathogen persistent transmission.Science advances · 2025Article
- Use of the 23S rRNA gene as a target template in the universal loop-mediated isothermal amplification (LAMP) of genomic DNA from phytoplasmas.Microbiology spectrum · 2024Article
- A Selective Bottleneck During Host Entry Drives the Evolution of New Legume Symbionts.Molecular biology and evolution · 2023Article
- Flavescence dorée phytoplasma enters insect cells by a clathrin-mediated endocytosis allowing infection of its insect vector.Scientific reports · 2023Article
- Article
- The Complete Genome of the "Flavescence Dorée" Phytoplasma Reveals Characteristics of Low Genome Plasticity.Biology · 2022Article
- Screening potential insect vectors in a museum biorepository reveals undiscovered diversity of plant pathogens in natural areas.Ecology and evolution · 2021Article
- Interactions between the flavescence dorée phytoplasma and its insect vector indicate lectin-type adhesion mediated by the adhesin VmpA.Scientific reports · 2021Article
- Phytoplasma diseases of plants: molecular diagnostics and way forward.World journal of microbiology & biotechnology · 2021Review
- Bacterial Vector-Borne Plant Diseases: Unanswered Questions and Future Directions.Molecular plant · 2020Review
- Ability ofInsects · 2020Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytoplasmas are transmitted by insect vectors in a persistent propagative manner; however, detailed movements and multiplication patterns of phytoplasmas within vectors remain elusive. In this study, spatiotemporal dynamics of onion yellows (OY) phytoplasma in its vector Macrosteles striifrons were investigated by immunohistochemistry-based 3D imaging, whole-mount fluorescence staining, and real-time quantitative PCR. The results indicated that OY phytoplasmas entered the anterior midgut epithelium by seven days after acquisition start (daas), then moved to visceral muscles surrounding the midgut and to the hemocoel at 14-21 daas; finally, OY phytoplasmas entered into type III cells of salivary glands at 21-28 daas. The anterior midgut of the alimentary canal and type III cells of salivary glands were identified as the major sites of OY phytoplasma infection. Fluorescence staining further revealed that OY phytoplasmas spread along the actin-based muscle fibers of visceral muscles and accumulated on the surfaces of salivary gland cells. This accumulation would be important for phytoplasma invasion into salivary glands, and thus for successful insect transmission. This study demonstrates the spatiotemporal dynamics of phytoplasmas in insect vectors. The findings from this study will aid in understanding of the underlying mechanism of insect-borne plant pathogen transmission.
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