ArticleNature communications2020
A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 121 citations in OpenAlex.
- Interaction Between PRDM14 and CBFA2T2 Supports Pluripotency and Proliferation in Germ Cell Tumors.Cancers · 2026Article
- NELFA supports naïve pluripotency and drives 8C-like state in human embryonic stem cells.Nature communications · 2026Article
- Mechanisms of human germ cell development.Nature reviews. Molecular cell biology · 2026Review
- Human-Mouse Chimerism for Organogenesis Without Neural or Germline Contributions.International journal of biological sciences · 2026Article
- The NANOS RNA-binding protein variants: a model for understanding human infertility.Journal of applied genetics · 2025Review
- Specialized signaling centers direct cell fate and spatial organization in a mesodermal organoid model.Science advances · 2025Article
- Single-cell transcriptomics of organoids reveals transcriptional control of germline stem cell fate by an E2F1-TFAP2C-SOX17 positive-feedback loop in Turner syndrome.Cell & bioscience · 2025Article
- Evolving Lessons on Metazoan Primordial Germ Cells in Diversity and Development.Molecular reproduction and development · 2025Review
- X-Linked Gene Dosage and SOX2 Act as Key Roadblocks for Human Germ Cell Specification in Klinefelter Syndrome.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The establishment and regulation of human germ cell lineage.Stem cell research & therapy · 2025Review
- Functional and molecular single-cell analyses implicate PRDM14 in the initiation of B cell leukemia in mice.Scientific reports · 2025Article
- Maternal PRDM10 activates essential genes for oocyte-to-embryo transition.Nature communications · 2025Article
- High levels of histone acetylation modifications promote the formation of PGCs.Poultry science · 2025Article
- DNA methylation in mammalian development and disease.Nature reviews. Genetics · 2025Review
- HELQ deficiency impairs the induction of primordial germ cell-like cells.FEBS open bio · 2024Article
- DDX5 Can Act as a Transcription Factor Participating in the Formation of Chicken PGCs by TargetingGenes · 2024Article
- Article
- PRDM14 extinction enables the initiation of trophoblast stem cell formation.Cellular and molecular life sciences : CMLS · 2024Article
- NANOG controls testicular germ cell tumour stemness through regulation of MIR9-2.Stem cell research & therapy · 2024Article
- Human primordial germ cell-like cells specified from resetting precursors develop in human hindgut organoids.Nature protocols · 2024Review
13 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
PRDM14 is a crucial regulator of mouse primordial germ cells (mPGCs), epigenetic reprogramming and pluripotency, but its role in the evolutionarily divergent regulatory network of human PGCs (hPGCs) remains unclear. Besides, a previous knockdown study indicated that PRDM14 might be dispensable for human germ cell fate. Here, we decided to use inducible degrons for a more rapid and comprehensive PRDM14 depletion. We show that PRDM14 loss results in significantly reduced specification efficiency and an aberrant transcriptome of hPGC-like cells (hPGCLCs) obtained in vitro from human embryonic stem cells (hESCs). Chromatin immunoprecipitation and transcriptomic analyses suggest that PRDM14 cooperates with TFAP2C and BLIMP1 to upregulate germ cell and pluripotency genes, while repressing WNT signalling and somatic markers. Notably, PRDM14 targets are not conserved between mouse and human, emphasising the divergent molecular mechanisms of PGC specification. The effectiveness of degrons for acute protein depletion is widely applicable in various developmental contexts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.