Evidence map›Paper›PMID 32152267›Full record

ArticleNature communications2020

Cytokinetic bridge triggers de novo lumen formation in vivo.

L I Rathbun, E G Colicino, J Manikas, J O'Connell, N Krishnan, N S Reilly, S Coyne, G Erdemci-Tandogan, A Garrastegui, J Freshour and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
4.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 47 citations in OpenAlex.

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  19. Morphogenetic Roles of Hydrostatic Pressure in Animal Development.Annual review of cell and developmental biology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

L I RathbunBiology Department, Syracuse University, Syracuse, New York, USA.
E G ColicinoBiology Department, Syracuse University, Syracuse, New York, USA.
J ManikasBiology Department, Syracuse University, Syracuse, New York, USA.
J O'ConnellBiology Department, Syracuse University, Syracuse, New York, USA.
N KrishnanBiology Department, Syracuse University, Syracuse, New York, USA.
N S ReillyDepartment of Physics and Astronomy, University of Rochester, Rochester, New York, USA.ORCID http://orcid.org/0000-0003-1491-8448
S CoyneDepartment of Cell and Developmental Biology, SUNY Upstate Medical School, Syracuse, New York, USA.
G Erdemci-TandoganDepartment of Physics, Syracuse University, Syracuse, New York, USA.
A GarrasteguiBiology Department, Syracuse University, Syracuse, New York, USA.
J FreshourBiology Department, Syracuse University, Syracuse, New York, USA.
P SantraDepartment of Cell and Developmental Biology, SUNY Upstate Medical School, Syracuse, New York, USA.
M L ManningDepartment of Physics, Syracuse University, Syracuse, New York, USA.
J D AmackDepartment of Cell and Developmental Biology, SUNY Upstate Medical School, Syracuse, New York, USA.ORCID http://orcid.org/0000-0002-5465-9754
H HehnlyBiology Department, Syracuse University, Syracuse, New York, USA. hhehnly@syr.edu.
Syracuse University · USSUNY Upstate Medical University · USUniversity of Rochester · US

Funding

The relationship between Rab11-endosomes and the centrosome during divisionR01GM127621 · NIGMS · SYRACUSE UNIVERSITY · PI HEHNLY, HEIDI · 2018 to 2022
$1.8M
Mother Centriole Appendages Regulate the AMIS Compartment and Cilogenesis.R00GM107355 · NIGMS · UPSTATE MEDICAL UNIVERSITY · PI HEHNLY, HEIDI · 2015 to 2017
$723k
NIGMS NIH HHS R00 GM107355NIGMS NIH HHS R01 GM127621
6 · The paper itself

Abstract

Multicellular rosettes are transient epithelial structures that serve as intermediates during diverse organ formation. We have identified a unique contributor to rosette formation in zebrafish Kupffer's vesicle (KV) that requires cell division, specifically the final stage of mitosis termed abscission. KV utilizes a rosette as a prerequisite before forming a lumen surrounded by ciliated epithelial cells. Our studies identify that KV-destined cells remain interconnected by cytokinetic bridges that position at the rosette's center. These bridges act as a landmark for directed Rab11 vesicle motility to deliver an essential cargo for lumen formation, CFTR (cystic fibrosis transmembrane conductance regulator). Here we report that premature bridge cleavage through laser ablation or inhibiting abscission using optogenetic clustering of Rab11 result in disrupted lumen formation. We present a model in which KV mitotic cells strategically place their cytokinetic bridges at the rosette center, where Rab11-associated vesicles transport CFTR to aid in lumen establishment.

Indexed as

AnimalsCell DivisionCell LineCell MovementCell PolarityCiliaCystic Fibrosis Transmembrane Conductance RegulatorEmbryonic DevelopmentEmbryo, NonmammalianEpithelial CellsGene Expression Regulation, DevelopmentalKupffer CellsMitosisOrganogenesisrab11 GTP-Binding Proteinsrab GTP-Binding ProteinsCFTR protein, zebrafishCystic Fibrosis Transmembrane Conductance Regulatorrab11 GTP-Binding Proteinsrab GTP-Binding ProteinsZebrafish Proteins

Identifiers

PMID32152267
PMCPMC7062744
OpenAlexW3009609165

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.