Evidence map›Paper›PMID 32152223›Full record

ArticleThe Journal of biological chemistry2020

Two- and three-color STORM analysis reveals higher-order assembly of leukotriene synthetic complexes on the nuclear envelope of murine neutrophils.

Angela B Schmider, Nicholas C Bauer, Hongjae Sunwoo, Matthew D Godin, Giorgianna E Ellis, Jeannie T Lee, Peter A Nigrovic, Roy J Soberman

Open access · hybridAbstract read
In one paragraph

Article in The Journal of biological chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Angela B SchmiderNephrology Division, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129.
Nicholas C BauerNephrology Division, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129.
Hongjae SunwooDepartment of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114.
Matthew D GodinNephrology Division, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129.
Giorgianna E EllisNephrology Division, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129.
Jeannie T LeeDepartment of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114; Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Peter A NigrovicDivision of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Roy J SobermanNephrology Division, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129. Electronic address: soberman@helix.mgh.harvard.edu.
Harvard University · USBrigham and Women's Hospital · USMassachusetts General Hospital · US

Funding

Joint Biology Consortium Resource-based CenterP30AR070253 · NIAMS · BRIGHAM AND WOMEN'S HOSPITAL · PI Peter A Nigrovic, Jeffrey Andrew Sparks · 2016 to 2026
$9.4M
TRAINING IN CELL STRUCTURE AND FUNCTION IN NEPHROLOGYT32DK007540 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI BROWN, DENNIS, SOBERMAN, ROY · 1986 to 2021
$7.2M
Role of B7-1 in Podocytes in Pathogenesis of ProteinuriaR01DK062472 · NIDDK · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI SOBERMAN, ROY · 2004 to 2017
$5.3M
Ly6 family members in neutrophil biologyR01AR065538 · NIAMS · BRIGHAM AND WOMEN'S HOSPITAL · PI NIGROVIC, PETER A · 2014 to 2024
$4.0M
The Macromolecular Organization of Leukotriene Synthesis and Renal InflammationK01DK089145 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI SCHMIDER, ANGELA BAIR · 2011 to 2015
$798k
Modulation of neutrophil function through emperipolesisR21AR076630 · NIAMS · BRIGHAM AND WOMEN'S HOSPITAL · PI NIGROVIC, PETER A · 2020 to 2021
$545k
Ly6 family members in neutrophil biologyR56AR065538 · NIAMS · BRIGHAM AND WOMEN'S HOSPITAL · PI NIGROVIC, PETER A · 2019 to 2019
$411k
A System for FLIM MicroscopyS10RR027931 · NCRR · MASSACHUSETTS GENERAL HOSPITAL · PI SOBERMAN, ROY · 2010 to 2010
$321k
NCRR NIH HHS S10 RR027931NIAMS NIH HHS P30 AR070253NIAMS NIH HHS R01 AR065538NIAMS NIH HHS R21 AR076630NIAMS NIH HHS R56 AR065538NIDDK NIH HHS K01 DK089145NIDDK NIH HHS R01 DK062472NIDDK NIH HHS T32 DK007540
6 · The paper itself

Abstract

Over the last several years it has become clear that higher order assemblies on membranes, exemplified by signalosomes, are a paradigm for the regulation of many membrane signaling processes. We have recently combined two-color direct stochastic optical reconstruction microscopy (dSTORM) with the (Clus-DoC) algorithm that combines cluster detection and colocalization analysis to observe the organization of 5-lipoxygenase (5-LO) and 5-lipoxygenase-activating protein (FLAP) into higher order assemblies on the nuclear envelope of mast cells; these assemblies were linked to leukotriene (LT) C

Indexed as

5-Lipoxygenase-Activating ProteinsAlgorithmsAnimalsArachidonate 5-LipoxygenaseCell NucleusCells, CulturedLeukotriene C4MiceMice, Inbred C57BLMicroscopyNeutrophilsOptical Imaging5-Lipoxygenase-Activating ProteinsArachidonate 5-LipoxygenaseLeukotriene C4cell biologycell signalingcytokinedirect stochastic optical reconstruction microscopy (dSTORM)leukotrieneneutrophilnuclear envelopesingle-molecule localization microscopysuperresolution microscopysupramolecular complex

Identifiers

PMID32152223
PMCPMC7186161
OpenAlexW3010574766

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.