Evidence map›Paper›PMID 32151748›Full record

ArticleNanomedicine : nanotechnology, biology, and medicine2020

Photoactivation of sulfonated polyplexes enables localized gene silencing by DsiRNA in breast cancer cells.

Anu Puri, Mathias Viard, Paul Zakrevsky, Serena Zampino, Arabella Chen, Camryn Isemann, Sohaib Alvi, Jeff Clogston, Upendra Chitgupi, Jonathan F Lovell and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nanomedicine : nanotechnology, biology, and medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Anu PuriRNA Structure and Design Section, RNA Biology Laboratory, National Cancer Institute, Frederick, MD, USA. Electronic address: puria@mail.nih.gov.
Mathias ViardRNA Structure and Design Section, RNA Biology Laboratory, National Cancer Institute, Frederick, MD, USA; Basic Science Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Paul ZakrevskyRNA Structure and Design Section, RNA Biology Laboratory, National Cancer Institute, Frederick, MD, USA.
Serena ZampinoRNA Structure and Design Section, RNA Biology Laboratory, National Cancer Institute, Frederick, MD, USA.
Arabella ChenRNA Structure and Design Section, RNA Biology Laboratory, National Cancer Institute, Frederick, MD, USA.
Camryn IsemannRNA Structure and Design Section, RNA Biology Laboratory, National Cancer Institute, Frederick, MD, USA.
Sohaib AlviRNA Structure and Design Section, RNA Biology Laboratory, National Cancer Institute, Frederick, MD, USA.
Jeff ClogstonBasic Science Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA; Nanotechnology Characterization Lab, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Upendra ChitgupiDepartment of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, NY, USA.
Jonathan F LovellDepartment of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, NY, USA.
Bruce A ShapiroRNA Structure and Design Section, RNA Biology Laboratory, National Cancer Institute, Frederick, MD, USA. Electronic address: shapirbr@mail.nih.gov.

Funding

Computational RNA NanodesignZIABC011061 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI SHAPIRO, BRUCE · 2009 to 2021
$12.3M
Light-Triggered Drug Release in Primed Pancreatic TumorsR01EB017270 · NIBIB · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI LOVELL, JONATHAN F · 2013 to 2021
$3.5M
Targeting Tumors with Resealable Nanovesicles Permeabilized by NIR LightDP5OD017898 · OD · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI LOVELL, JONATHAN F · 2013 to 2017
$1.9M
CCR NIH HHS HHSN261200800001CIntramural NIH HHS ZIA BC011061NCI NIH HHS HHSN261200800001ENIBIB NIH HHS R01 EB017270NIH HHS DP5 OD017898
6 · The paper itself

Abstract

Translation potential of RNA interference nanotherapeutics remains challenging due to in vivo off-target effects and poor endosomal escape. Here, we developed novel polyplexes for controlled intracellular delivery of dicer substrate siRNA, using a light activation approach. Sulfonated polyethylenimines covalently linked to pyropheophorbide-α for photoactivation and bearing modified amines (sulfo-pyro-PEI) for regulated endosomal escape were investigated. Gene knock-down by the polymer-complexed DsiRNA duplexes (siRNA-NPs) was monitored in breast cancer cells. Surprisingly, sulfo-pyro-PEI/siRNA-NPs failed to downregulate the PLK1 or eGFP proteins. However, photoactivation of these cell associated-polyplexes with a 661-nm laser clearly restored knock-down of both proteins. In contrast, protein down-regulation by non-sulfonated pyro-PEI/siRNA-NPs occurred without any laser treatments, indicating cytoplasmic disposition of DsiRNA followed a common intracellular release mechanism. Therefore, sulfonated pyro-PEI holds potential as a unique trap and release light-controlled delivery platform for on-demand gene silencing bearing minimal off target effects.

Indexed as

Gene SilencingBreast NeoplasmsCell Cycle ProteinsCell Line, TumorDEAD-box RNA HelicasesEndosomesFemaleGene Knockdown TechniquesGreen Fluorescent ProteinsHumansPolo-Like Kinase 1PolyethyleneiminePolymersProtein Serine-Threonine KinasesProto-Oncogene ProteinsRibonuclease IIICell Cycle ProteinsDEAD-box RNA HelicasesDICER1 protein, humanenhanced green fluorescent proteinGreen Fluorescent ProteinsPolo-Like Kinase 1PolyethyleneiminePolymersProtein Serine-Threonine KinasesProto-Oncogene ProteinsRibonuclease IIIRNA, Small InterferingEndosomal escapePhotosensitizerPolymerRNA interferencesiRNA delivery

Identifiers

PMID32151748
PMCPMC8117728

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.