ArticlePolymers2020
Bovine Decellularized Amniotic Membrane: Extracellular Matrix as Scaffold for Mammalian Skin.
Article in Polymers, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Experimental esophageal reconstruction in dogs using autologous platelet-rich plasma-augmented acellular amniotic membrane scaffold.Open veterinary journal · 2026Article
- Human Amniotic Membrane Procurement Protocol and Evaluation of a Simplified Alkaline Decellularization Method.Methods and protocols · 2026Article
- Bioengineered Tricomposite Hydrogel Enhances Chondrogenic Phenotype and Hyaline Matrix Formation in Human Chondrocytes.Gels (Basel, Switzerland) · 2025Article
- Therapeutic Potential of Bovine Amniotic Membrane in Wound Healing: Insights from a Mouse Model.Cells · 2025Article
- A Review on Bioengineering the Bovine Mammary Gland: The Role of the Extracellular Matrix and Reconstruction Prospects.Bioengineering (Basel, Switzerland) · 2025Review
- Recent advances in bionic scaffolds for cartilage tissue engineering.Frontiers in bioengineering and biotechnology · 2025Review
- Acellular porcine placental membranes as a novel biomaterial for tissue repair applications.Frontiers in bioengineering and biotechnology · 2025Article
- Viewing Decellularized Amniotic Membrane Through the Lens of Coupled Scaffolding and Drug Delivery Systems in Regenerative Medicine.Journal of tissue engineering and regenerative medicine · 2025Review
- Decellularization of various tissues and organs through chemical methods.Tissue & cell · 2024Review
- 3D morphometry of endothelial cells angiogenesis in an extracellular matrix composite hydrogel.Heliyon · 2024Article
- Femtosecond Laser Cutting of Human Crystalline Lens Capsule and Decellularization for Corneal Endothelial Bioengineering.Bioengineering (Basel, Switzerland) · 2024Article
- Decellularization and Their Significance for Tissue Regeneration in the Era of 3D Bioprinting.ACS omega · 2024Review
- Recent Advances in Decellularized Matrix-Derived Materials for Bioink and 3D Bioprinting.Gels (Basel, Switzerland) · 2023Review
- Decellularized Vascular Scaffolds Derived from Bovine Placenta Blood Vessels.Arquivos brasileiros de cardiologia · 2023Article
- Mimicking the Natural Basement Membrane for Advanced Tissue Engineering.Biomacromolecules · 2022Review
- Cellular Therapeutics for Chronic Wound Healing: Future for Regenerative Medicine.Current drug targets · 2022Review
- Rational Design of Immunomodulatory Hydrogels for Chronic Wound Healing.Advanced materials (Deerfield Beach, Fla.) · 2021Review
- Polymer Biointerfaces.Polymers · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Decellularized membranes (DM) were obtained from bovine amniotic membranes (BAM) using four different decellularization protocols, based on physical, chemical, and mechanical treatment. The new material was used as a biological scaffold for in vitro skin cell culture. The DM were characterized using hematoxylin-eosin assay, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR-ATR), and differential scanning calorimetry (DSC). The in vitro cytotoxicity of DM was evaluated using MTT. The efficacy of decellularization process was assessed through DNA quantification and electrophoresis. All the used protocols showed a high effectiveness in terms of elimination of native cells, confirmed by DNA extraction and quantification, electrophoresis, and SEM, although protocol IV removes the cellular contents and preserve the native extracellular matrix (ECM) architecture which it can be considered as the most effective in terms of decellularization. FTIR-ATR and DSC on the other hand, revealed the effects of decellularization on the biochemical composition of the matrices. There was no cytotoxicity and the biological matrices obtained were a source of collagen for recellularization. The matrices of protocols I, II, and III were degraded at day 21 of cell culture, forming a gel. The biocompatibility in vitro was demonstrated; hence these matrices may be deemed as potential scaffold for epithelial tissue regeneration.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.