ArticleAntioxidants (Basel, Switzerland)2020
Vitamin C Activates the Folate-Mediated One-Carbon Cycle in C2C12 Myoblasts.
Article in Antioxidants (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- A Comprehensive Review of Fortification, Bioavailability, and Health Benefits of Folate.International journal of molecular sciences · 2025Review
- Vitamin C: From Self-Sufficiency to Dietary Dependence in the Framework of Its Biological Functions and Medical Implications.Life (Basel, Switzerland) · 2025Review
- Differences in honeybee queen pheromones revealed by LC-MS/MS: Reassessing the honest signal hypothesis.iScience · 2024Article
- Glutathione-mediated redox regulation in Cryptococcus neoformans impacts virulence.Nature microbiology · 2024Article
- Placenta-Related Parameters at Delivery in Relation to Folic Acid Supplementation in Different Pregnancies.Nutrients · 2024Article
- Phosphate availability conditions caspofungin tolerance, capsule attachment and titan cell formation inFrontiers in fungal biology · 2024Article
- Article
- A protective maternal nutrient concomitant intake associated with acute leukemia might be modified by sex, in children under 2 years.Frontiers in oncology · 2023Article
- Vitamin C and folate status in hereditary fructose intolerance.European journal of clinical nutrition · 2022Article
- Ascorbate as a Bioactive Compound in Cancer Therapy: The Old Classic Strikes Back.Molecules (Basel, Switzerland) · 2022Review
- Vitamin Supplements as a Nutritional Strategy against Chronic Alcohol Consumption? An Updated Review.Antioxidants (Basel, Switzerland) · 2022Review
- Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation.International journal of biological sciences · 2022Article
- The Impact of the hAPP695SW Transgene and Associated Amyloid-β Accumulation on Murine Hippocampal Biochemical Pathways.Journal of Alzheimer's disease : JAD · 2022Article
- Xanthohumol Pyrazole Derivative Improves Diet-Induced Obesity and Induces Energy Expenditure in High-Fat Diet-Fed Mice.ACS pharmacology & translational science · 2021Article
- Article
- Antioxidants in Potatoes: A Functional View on One of the Major Food Crops Worldwide.Molecules (Basel, Switzerland) · 2021Review
- Article
- Modulators of Oxidative Stress: Chemical and Pharmacological Aspects.Antioxidants (Basel, Switzerland) · 2020Article
- Cadmium-Induced Oxidative Stress: Focus on the Central Nervous System.Antioxidants (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Vitamin C (L-ascorbic acid, AA) is an essential cellular antioxidant and cofactor for several α-ketoglutarate-dependent dioxygenases. As an antioxidant, AA interacts with vitamin E to control oxidative stress. While several reports suggest an interaction of AA with folate (vitamin B9) in animals and humans, little is known about the nature of the interaction and the underlying molecular mechanisms at the cellular level. We used an untargeted metabolomics approach to study the impact of AA on the metabolome of C2C12 myoblast cells. Compared to untreated cells, treatment of C2C12 cells with AA at 100 µM resulted in enhanced concentrations of folic acid (2.5-fold) and 5-methyl-tetrahydrofolate (5-methyl-THF, 10-fold increase) whereas the relative concentrations of 10-formyl-tetrahydrofolate decreased by >90% upon AA pretreatment, indicative of increased utilization for the biosynthesis of active THF metabolites. The impact of AA on the folate-mediated one-carbon cycle further manifested itself as an increase in the levels of methionine, whose formation from homocysteine is 5-methyl-THF dependent, and an increase in thymidine, whose formation from deoxyuridine monophosphate (dUMP) is dependent on 5,10-methylene-THF. These findings shed new light on the interaction of AA with the folate-mediated one-carbon cycle and partially explain clinical findings that AA supplementation enhances erythrocyte folate status and that it may decrease serum levels of homocysteine, which is considered as a biomarker of cardiovascular disease risk.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.