ArticlePloS one2020
An optimised saliva collection method to produce high-yield, high-quality RNA for translational research.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Assessment of salivary matrix metalloproteinase (MMP8) and activated salivary matrix metalloproteinase (aMMP8) in periodontitis patients: a systematic review and meta-analysis.Frontiers in oral health · 2025Pooled it
- Longitudinal profiling of upper respiratory tract microbiota and metabolome in hospitalized COVID-19 convalescents: a 3-year prospective cohort study.Journal of translational medicine · 2026Article
- Characterizing Human Milk RNA Degradation Over Time to Optimize Storage of Human Milk for RNA Quality.Journal of mammary gland biology and neoplasia · 2026Article
- Occlusal types shape oral microbiome stomatotypes and metabolic landscapes: A multi-omics perspective on host-microbe interaction.Microbial cell (Graz, Austria) · 2026Article
- Review
- Exploring the potential of salivary small RNAs as non-invasive biomarkers in pigs.Journal of animal science and technology · 2025Article
- Comprehensive integrative analysis of circadian rhythms in human saliva.Npj biological timing and sleep · 2025Article
- Salivary Extracellular Vesicles Separation: Analysis of Ultracentrifugation-Based Protocols.Oral diseases · 2025Article
- The Salivary Transcriptome: A Window into Local and Systemic Gene Expression Patterns.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Article
- Characterization of non-invasive oropharyngeal samples and nucleic acid isolation for molecular diagnostics.Scientific reports · 2024Article
- Development of an integrated sample amplification control for salivary point-of-care pathogen testing.Analytica chimica acta · 2024Article
- Salivary diagnostics: opportunities and challenges.Theranostics · 2024Review
- Assessing the impact of storage conditions on RNA from human saliva and its application to the identification of mRNA biomarkers for asthma.Frontiers in molecular biosciences · 2024Article
- Extrinsic and intrinsic preanalytical variables affecting liquid biopsy in cancer.Cell reports. Medicine · 2023Review
- Obtaining miRNA from Saliva-Comparison of Sampling and Purification Methods.International journal of molecular sciences · 2023Article
- Promising applications of human-derived saliva biomarker testing in clinical diagnostics.International journal of oral science · 2023Review
- Comparing SARS-CoV-2 Viral Load in Human Saliva to Oropharyngeal Swabs, Nasopharyngeal Swabs, and Sputum: A Systematic Review and Meta-Analysis.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2023Review
- Article
- Sample Buffer Containing Guanidine-Hydrochloride Combines Biological Safety and RNA Preservation for SARS-CoV-2 Molecular Diagnostics.Diagnostics (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
16 authors.
Funding
Abstract
Saliva represents an ideal matrix for diagnostic biomarker development as it is readily available and requires no invasive collection procedures. However, salivary RNA is labile and rapidly degrades. Previous attempts to isolate RNA from saliva have yielded poor quality and low concentrations. Here we compare collection and processing methods and propose an approach for future studies. The effects of RNA stabilisers, storage temperatures, length of storage and fasting windows were investigated on pooled saliva samples from healthy volunteers. Isolated RNA was assessed for concentration and quality. Bacterial growth was investigated through RT-PCR using bacterial and human primers. Optimal conditions were implemented and quality controlled in a clinical setting. The addition of RNAlater increased mean RNA yield from 4912 ng/μl to 15,473 ng and RNA Integrity Number (RIN) from 4.5 to 7.0. No significant changes to RNA yield were observed for storage at room temperature beyond 1 day or at -80 °C. Bacterial growth did not occur in samples stored at ambient temperature for up to a week. There was a trend towards higher RNA concentration when saliva was collected after overnight fasting but no effect on RIN. In the clinic, RNA yields of 6307 ng and RINs of 3.9 were achieved, improving on previous reports. The method we describe here is a robust, clinically feasible saliva collection method using preservative that gives high concentrations and improved RINs compared to saliva collected without preservative.
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Registered trials
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