Evidence map›Paper›PMID 32150579›Full record

ArticlePloS one2020

Apparent size and morphology of bacterial microcompartments varies with technique.

Nolan W Kennedy, Jasmine M Hershewe, Taylor M Nichols, Eric W Roth, Charlene D Wilke, Carolyn E Mills, Michael C Jewett, Danielle Tullman-Ercek

Abstract read
In one paragraph

Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Review
  13. Variety of size and form of GRM2 bacterial microcompartment particles.Protein science : a publication of the Protein Society · 2021
    Article
  14. Advances in the World of Bacterial Microcompartments.Trends in biochemical sciences · 2021
    Review
  15. Article
  16. Article
  17. Review
  18. Article
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Nolan W KennedyInterdisciplinary Biological Sciences Graduate Program, Northwestern University, Evanston, Illinois, United States of America.ORCID 0000-0002-2944-5439
Jasmine M HersheweDepartment of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, United States of America.ORCID 0000-0003-4795-9721
Taylor M NicholsDepartment of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, United States of America.ORCID 0000-0002-2342-9681
Eric W RothNorthwestern University Atomic and Nanoscale Characterization Experimentation Center, Northwestern University, Evanston, Illinois, United States of America.
Charlene D WilkeNorthwestern University Atomic and Nanoscale Characterization Experimentation Center, Northwestern University, Evanston, Illinois, United States of America.
Carolyn E MillsDepartment of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, United States of America.ORCID 0000-0001-6058-0762
Michael C JewettDepartment of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, United States of America.
Danielle Tullman-ErcekDepartment of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, United States of America.ORCID 0000-0001-6734-480X

Funding

Developing Capacity to Evaluate Training Programs via Development of Human, Institutional and Social CapitalT32GM008449 · NIGMS · NORTHWESTERN UNIVERSITY · PI LEONARD, JOSHUA NATHANIEL · 1993 to 2023
$7.8M
NIGMS NIH HHS T32 GM008449
6 · The paper itself

Abstract

Bacterial microcompartments (MCPs) are protein-based organelles that encapsulate metabolic pathways. Metabolic engineers have recently sought to repurpose MCPs to encapsulate heterologous pathways to increase flux through pathways of interest. As MCP engineering becomes more common, standardized methods for analyzing changes to MCPs and interpreting results across studies will become increasingly important. In this study, we demonstrate that different imaging techniques yield variations in the apparent size of purified MCPs from Salmonella enterica serovar Typhimurium LT2, likely due to variations in sample preparation methods. We provide guidelines for preparing samples for MCP imaging and outline expected variations in apparent size and morphology between methods. With this report we aim to establish an aid for comparing results across studies.

Indexed as

Gene Expression Regulation, BacterialMetabolic Networks and PathwaysSalmonella typhimurium

Identifiers

PMID32150579
PMCPMC7062276

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.