ArticleeLife2020
Fully automated, sequential focused ion beam milling for cryo-electron tomography.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
59 citing papers in PubMed.
- Sphingolipids associate with the chlamydial nucleoid and mark developmental transitions in Chlamydia trachomatis.Nature communications · 2026Article
- CryoGO enables high-resolution structural profiling of endogenous cellular macromolecules.bioRxiv : the preprint server for biology · 2026Article
- Article
- Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET.Nature protocols · 2026Review
- In situ structure determination of conformationally flexible targets using nextPYP.Nature protocols · 2026Review
- Reproducible Sample Preparation of Virus-Infected Cells for Cryo-FIB/ET Using Manual Plunge Freezing.Bio-protocol · 2026Article
- Structure and assembly of the A-C linker connecting microtubule triplets in centrioles.Science advances · 2025Article
- High-throughput cryo-electron tomography enables multiscale visualization of the inner life of microbes.Current opinion in structural biology · 2025Review
- RNA sample optimization for cryo-EM analysis.Nature protocols · 2025Review
- A case for community metadata standards in cryo-electron tomography.Emerging topics in life sciences · 2025Review
- Xenon plasma focused ion beam lamella fabrication on high-pressure frozen specimens for structural cell biology.Nature communications · 2025Article
- In situ and in vitro cryo-EM reveal structures of mycobacterial encapsulin assembly intermediates.Communications biology · 2025Article
- Structural biology inside multicellular specimens using electron cryotomography.Quarterly reviews of biophysics · 2025Review
- Thermophilic bacteria employ a contractile injection system in hot spring microbial mats.The ISME journal · 2025Article
- Cryo-electron tomography: en route to the molecular anatomy of organisms and tissues.Biochemical Society transactions · 2024Review
- Visualizing the virus world inside the cell by cryo-electron tomography.Journal of virology · 2024Review
- Recent advances in correlative cryo-light and electron microscopy.Current opinion in structural biology · 2024Review
- Archaeal type six secretion system mediates contact-dependent antagonism.Science advances · 2024Article
- Cell envelope diversity and evolution across the bacterial tree of life.Nature microbiology · 2024Review
- Smart parallel automated cryo-electron tomography.Nature methods · 2024Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Cryo-electron tomography (cryoET) has become a powerful technique at the interface of structural biology and cell biology, due to its unique ability for imaging cells in their native state and determining structures of macromolecular complexes in their cellular context. A limitation of cryoET is its restriction to relatively thin samples. Sample thinning by cryo-focused ion beam (cryoFIB) milling has significantly expanded the range of samples that can be analyzed by cryoET. Unfortunately, cryoFIB milling is low-throughput, time-consuming and manual. Here, we report a method for fully automated sequential cryoFIB preparation of high-quality lamellae, including rough milling and polishing. We reproducibly applied this method to eukaryotic and bacterial model organisms, and show that the resulting lamellae are suitable for cryoET imaging and subtomogram averaging. Since our method reduces the time required for lamella preparation and minimizes the need for user input, we envision the technique will render previously inaccessible projects feasible.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.