ArticleIUCrJ2020
Comparing serial X-ray crystallography and microcrystal electron diffraction (MicroED) as methods for routine structure determination from small macromolecular crystals.
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Article in IUCrJ, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Application of electron diffraction to rapid structural elucidation of crude reaction products.Communications chemistry · 2026Article
- Advances of 3D microcrystals electron diffraction for transmembrane protein structure determination.Biophysical journal · 2026Review
- Multimodal Nanoscale Mapping of Local Structure and COJournal of the American Chemical Society · 2026Article
- Review
- MicroED: Unveiling the Structural Chemistry of Plant Biomineralisation.Molecules (Basel, Switzerland) · 2024Article
- Real-time observation of a metal complex-driven reaction intermediate using a porous protein crystal and serial femtosecond crystallography.Nature communications · 2024Article
- 3D Nanocrystallography and the Imperfect Molecular Lattice.Annual review of physical chemistry · 2024Review
- Mapping protein dynamics at high spatial resolution with temperature-jump X-ray crystallography.Nature chemistry · 2023Article
- Structural insights into functional properties of the oxidized form of cytochrome c oxidase.Nature communications · 2023Article
- Modular droplet injector for sample conservation providing new structural insight for the conformational heterogeneity in the disease-associated NQO1 enzyme.Lab on a chip · 2023Article
- Electron Diffraction of 3D Molecular Crystals.Chemical reviews · 2022Review
- Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy.Annual review of biochemistry · 2022Review
- Review
- Finding order in chaos - nanocrystals in amorphous protein gels.Acta crystallographica. Section F, Structural biology communications · 2021Article
- MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography.Nature communications · 2021Article
- A new electron diffraction approach for structure refinement applied to CaActa crystallographica. Section A, Foundations and advances · 2021Article
- Macromolecular crystallography using microcrystal electron diffraction.Acta crystallographica. Section D, Structural biology · 2021Review
- MicroED Sample Preparation and Data Collection For Protein Crystals.Methods in molecular biology (Clifton, N.J.) · 2021Article
- MicroED structure of lipid-embedded mammalian mitochondrial voltage-dependent anion channel.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures.Journal of applied crystallography · 2020Article
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Authors and funding
38 authors.
Alexander M WolffGraduate Program in Biophysics, University of California, San Francisco, San Francisco, California, USA.ORCID https://orcid.org/0000-0003-0474-7673
Iris D YoungDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.
Raymond G SierraLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Aaron S BrewsterMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Michael W MartynowyczHoward Hughes Medical Institute, University of California, Los Angeles, Los Angeles, California, USA.
Eriko NangoRIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.ORCID https://orcid.org/0000-0001-9851-7355
Michihiro SugaharaRIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
Takanori NakaneDepartment of Biological Science, Graduate School of Science, The University of Tokyo, Tokyo, Japan.ORCID https://orcid.org/0000-0003-2697-2767
Kazutaka ItoDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.
Andrew AquilaLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Asmit BhowmickMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Justin T BielGraduate Program in Biophysics, University of California, San Francisco, San Francisco, California, USA.
Sergio CarbajoLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.ORCID https://orcid.org/0000-0002-5292-4470
Aina E CohenSSRL, SLAC National Accelerator Laboratory, Menlo Park, California, USA.ORCID https://orcid.org/0000-0003-2414-9427
Saul CortezDepartment of Biology, San Francisco State University, San Francisco, California, USA.ORCID https://orcid.org/0000-0002-6820-0936
Ana GonzalezSSRL, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Tomoya HinoDepartment of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, 4-101 Koyama-cho, Minami, Tottori 680-8552, Japan.
Dohyun ImDepartment of Cell Biology, Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
Jake D KoralekLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Minoru KuboRIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
Tomas S LazarouDepartment of Chemistry, New York University, New York, USA.
Takashi NomuraRIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
Shigeki OwadaRIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.ORCID https://orcid.org/0000-0002-1451-7612
Avi J SamelsonInstitute for Neurodegenerative Diseases, University of California, San Francisco, San Francisco, California, USA.
Tomoyuki TanakaRIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
Rie TanakaRIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.ORCID https://orcid.org/0000-0003-1062-4792
Erin M ThompsonDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.
Henry van den BedemBioscience Department, SLAC National Accelerator Laboratory, Menlo Park, California, USA.ORCID https://orcid.org/0000-0003-2358-841X
Rahel A WoldeyesDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.
Fumiaki YumotoStructural Biology Research Center, Institute of Materials Structure Science, KEK/High Energy Accelerator Research Organization, Tsukuba, Ibaraki 305-0034, Japan.ORCID https://orcid.org/0000-0002-9592-397X
Wei ZhaoDepartment of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
Kensuke TonoRIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.ORCID https://orcid.org/0000-0003-1218-3759
Sebastien BoutetLinac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.ORCID https://orcid.org/0000-0003-3928-1244
So IwataRIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
Tamir GonenHoward Hughes Medical Institute, University of California, Los Angeles, Los Angeles, California, USA.
Nicholas K SauterMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
James S FraserDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.ORCID https://orcid.org/0000-0002-5080-2859
Michael C ThompsonDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.ORCID https://orcid.org/0000-0002-6099-2027
Funding
STRUCTURAL BIOLOGY TRAINING PROGRAMT32GM008284 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KORTEMME, TANJA · 1988 to 2022
$10.1MRESEARCH TRAINING IN CHEMISTRY AND CHEMICAL BIOLOGYT32GM064337 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CRAIK, CHARLES SCOTT · 2002 to 2021
$7.8MDIALS: New Computational Methods to Enable Challenging Crystallographic ExperimentsR01GM117126 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI SAUTER, NICHOLAS K · 2016 to 2023
$6.1MEliminating Critical Systematic Errors In Structural Biology With Next-Generation SimulationR01GM124149 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HOLTON, JAMES M · 2017 to 2024
$2.5MSupplement to Resolving ensemble averaged conformations by multi-temperature x-ray crystallography - Equipment 2020R01GM123159 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FRASER, JAMES SOLOMON · 2018 to 2021
$2.3MBiophysical rescue of Coagulation Factor IXa conformational ensembles from hemophilia B disease mutationsF32HL129989 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI THOMPSON, MICHAEL C. · 2015 to 2017
$168kNHLBI NIH HHS F32 HL129989NIGMS NIH HHS R01 GM117126NIGMS NIH HHS R01 GM123159NIGMS NIH HHS R01 GM124149NIGMS NIH HHS T32 GM008284NIGMS NIH HHS T32 GM064337
Abstract
Innovative new crystallographic methods are facilitating structural studies from ever smaller crystals of biological macromolecules. In particular, serial X-ray crystallography and microcrystal electron diffraction (MicroED) have emerged as useful methods for obtaining structural information from crystals on the nanometre to micrometre scale. Despite the utility of these methods, their implementation can often be difficult, as they present many challenges that are not encountered in traditional macromolecular crystallography experiments. Here, XFEL serial crystallography experiments and MicroED experiments using batch-grown microcrystals of the enzyme cyclophilin A are described. The results provide a roadmap for researchers hoping to design macromolecular microcrystallography experiments, and they highlight the strengths and weaknesses of the two methods. Specifically, we focus on how the different physical conditions imposed by the sample-preparation and delivery methods required for each type of experiment affect the crystal structure of the enzyme.
Indexed as
batch crystallizationmicrocrystalsMicroEDserial crystallography
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