ArticleJournal of fluorescence2002
Enhanced Emission Induced by FRET from a Long-Lifetime, Low Quantum Yield Donor to a Long-Wavelength, High Quantum Yield Acceptor.
Article in Journal of fluorescence, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Intermolecular dark resonance energy transfer (DRET) for high contrast imaging of endogenous mRNAs in 3D biological samples.Scientific reports · 2025Article
- Advances in FRET methodologies for probing molecular interactions.Biophysical journal · 2025Article
- Direct Observation of Triplet-Triplet Energy Transfer in DNA between Energy Donor and Acceptor C‑Nucleotides.JACS Au · 2025Article
- Long-distance tmFRET using bipyridyl- and phenanthroline-based ligands.Biophysical journal · 2024Article
- The Optimization of the One-Pot Synthesis of Au@SiOMaterials (Basel, Switzerland) · 2024Article
- Long-distance tmFRET using bipyridyl- and phenanthroline-based ligands.bioRxiv : the preprint server for biology · 2024Article
- Ruthenium(II) Polypyridyl Complexes as FRET Donors: Structure- and Sequence-Selective DNA-Binding and Anticancer Properties.Journal of the American Chemical Society · 2023Article
- Quenching of Protein Fluorescence by Fullerenol CInternational journal of molecular sciences · 2022Article
- Upconversion FRET quantitation: the role of donor photoexcitation mode and compositional architecture on the decay and intensity based responses.Light, science & applications · 2022Article
- Resonance energy transfer between fluorescent BSA protected Au nanoclusters and organic fluorophores.Nanoscale · 2014Article
- Non-natural amino acid fluorophores for one- and two-step fluorescence resonance energy transfer applications.Analytical biochemistry · 2010Article
- Annealed silver-island films for applications in metal-enhanced fluorescence: interpretation in terms of radiating plasmons.Journal of fluorescence · 2005Article
- Radiative decay engineering 5: metal-enhanced fluorescence and plasmon emission.Analytical biochemistry · 2005Article
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3 authors.
Funding
Abstract
We report observation of high quantum yield, long-lifetime fluorescence from a red dye BO-PRO-3 excited by resonance energy transfer (RET). The acceptor fluorescence was highly enhanced upon binding to the donor-labeled DNA. A ruthenium complex (Ru) was chosen as a donor in this system because of its long fluorescence lifetime. Both donor and acceptor were non-covalently bound to DNA. Emission from the donor-acceptor system (DA) at wavelengths exceeding 600 nm still preserves the long-lifetime component of the Ru donor, retaining average fluorescence lifetimes in the range of 30-50 ns. Despite the low quantum yield of the Ru donor in the absence of acceptor, its overall quantum yield of the DA pair was increased by energy transfer to the higher quantum yield acceptor BO-PRO-3. The wavelength-integrated intensity of donor and acceptor bound to DNA was many-fold greater than the intensity of the donor and acceptor separately bound to DNA. The origin of this effect is due to an efficient energy transfer from the donor, competing with non-radiative depopulation of the donor excited state. The distinctive features of DA complexes can be used in the development of a new class of engineered luminophores that display both long lifetime and long-wavelength emission. Similar DA complexes can be applied as proximity indicators, exhibiting strong fluorescence of adjacently located donors and acceptors over the relatively weak fluorescence of separated donors and acceptors.
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