ArticleClinical and experimental reproductive medicine2020
The effect of vitamin C on the gene expression profile of sperm protamines in the male partners of couples with recurrent pregnancy loss: A randomized clinical trial.
Article in Clinical and experimental reproductive medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 2 syntheses or guidelines pooled it, 24 citations in OpenAlex.
- Effects of vitamin E and vitamin C on male infertility: a meta-analysis.International urology and nephrology · 2022Pooled it
- Evaluation of the association between polymorphisms of PRM1 and PRM2 and the risk of male infertility: a systematic review, meta-analysis, and meta-regression.Scientific reports · 2020Pooled it
- Nutritional modulation of male fertility: a molecular-focused scoping review.Journal of health, population, and nutrition · 2026Article
- Therapeutic effects ofInternational journal of reproductive biomedicine · 2026Article
- Redox-Genomic Crosstalk: Linking Oxidative Stress, Sperm DNA Fragmentation, and Epigenetics in Personalized Management of Male Infertility.Journal of personalized medicine · 2026Review
- Is there a correlation between male partner's previous tuberculosis and recurrent pregnancy loss (RPL)? - a single-center retrospective analysis.Open medicine (Warsaw, Poland) · 2026Article
- Moringa oleifera Lam. As a Herbal Antioxidant-Enriched Semen Extender in Cryopreservation: A Critical Review of Its Protective Potential.Veterinary medicine and science · 2026Review
- Vitamin C as a Cardioprotective Agent Against Doxorubicin-Induced Cardiotoxicity.Journal of the American Heart Association · 2025Review
- Use of Natural Biomolecules in Animal Feed to Enhance Livestock Reproduction.International journal of molecular sciences · 2025Review
- Effects of Low Vitamin C Intake on Fertility Parameters and Pregnancy Outcomes in Guinea Pigs.Nutrients · 2023Article
- Male Contributory Factors in Recurrent Pregnancy Loss.Reproductive sciences (Thousand Oaks, Calif.) · 2023Review
- In Vitro Combination of Ascorbic and Ellagic Acids in Sperm Oxidative Damage Inhibition.International journal of molecular sciences · 2022Article
- Impact of Vitamin C on Gene Expression Profile of Inflammatory and Anti-Inflammatory Cytokines in the Male Partners of Couples with Recurrent Pregnancy Loss.International journal of inflammation · 2022Article
- What is known so far about bull sperm protamination: a review.Animal reproduction · 2022Review
- Utility of Antioxidants in the Treatment of Male Infertility: Clinical Guidelines Based on a Systematic Review and Analysis of Evidence.The world journal of men's health · 2021Review
- Vitamins as primary or adjunctive treatment in infertile men with varicocele: A systematic review.Arab journal of urology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveSince sperm abnormalities are known to be a major reason for recurrent pregnancy loss (RPL), any defects in DNA structure and chromatin condensation can place embryos at risk in the early stage of development and implantation. As antioxidants such as vitamin C may play a protective role against the destruction of protamine genes in sperm chromatin, this study was conducted to evaluate the effects of vitamin C on chromatin and the expression of protamine genes in the male partners of couples with RPL.
methodsTwenty male partners of couples with RPL were selected as the intervention group and received vitamin C supplementation (250 mg daily for 3 months). Healthy fertile men (n=20) were included as controls. Sperm chromatin, DNA integrity, and the expression levels of protamine genes were evaluated before and after treatment.
resultsSignificant differences were found in sperm morphology, protamine deficiency, and apoptosis between the two groups and before and after vitamin C administration. A significant change was found in mRNA levels of PRM1, PRM2, and the PRM1/PRM2 ratio after treatment.
conclusionDaily oral administration of vitamin C may improve human sperm parameters and DNA integrity by increasing protamine gene expression levels in the male partners of couples with RPL. The beneficial effects of vitamin C supplementation as an antioxidant for the male partners of couples with RPL could lead to improved pregnancy outcomes in these cases.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.