Evidence map›Paper›PMID 32144663›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Methods to Study the Structure and Catalytic Activity of cis-Splicing Inteins.

Jing Zhao, Zhenming Du, Chunyu Wang, Kenneth V Mills

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 96% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Jing ZhaoCenter for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA.
Zhenming DuDepartment of Chemistry, Georgia State University, Atlanta, GA, USA.
Chunyu WangCenter for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA.
Kenneth V MillsDepartment of Chemistry, College of the Holy Cross, Worcester, MA, USA. kmills@holycross.edu.
Rensselaer Polytechnic Institute · USCollege of the Holy Cross · USGeorgia State University · US

Funding

Structural Mechanisms of Hedgehog Autoprocessing in Physiology and DiseaseR01CA206592 · NCI · RENSSELAER POLYTECHNIC INSTITUTE · PI CALLAHAN, BRIAN PATRICK, WANG, CHUNYU · 2017 to 2021
$2.0M
Predoctoral Training Program for Alzheimer’s Disease at the Interface of Data Science, Engineering and BiologyT32AG078123 · NIA · RENSSELAER POLYTECHNIC INSTITUTE · PI Chunyu Wang · 2023 to 2026
$974k
The Regulation of Inteins: Adaptations in Extremophiles and Targets for Drug DiscoveryR15GM132817 · NIGMS · COLLEGE OF THE HOLY CROSS · PI MILLS, KENNETH V · 2019 to 2024
$723k
NCI NIH HHS R01 CA206592NIA NIH HHS T32 AG078123NIGMS NIH HHS R15 GM132817
6 · The paper itself

Abstract

The autocatalytic process of protein splicing is facilitated by an intein, which interrupts flanking polypeptides called exteins. The mechanism of protein splicing has been studied by overexpression in E. coli of intein fusion proteins with nonnative exteins. Inteins can be used to generate reactive α-thioesters, as well as proteins with N-terminal Cys residues, to facilitate expressed protein ligation. As such, a more detailed understanding of the function of inteins can have significant impact for biotechnology applications. Here, we provide biochemical methods to study splicing activity and NMR methods to study intein structure and the catalytic mechanism.

Indexed as

BiocatalysisInteinsProtein SplicingAspartic AcidBiochemistryCarbon IsotopesCysteineElectrophoresis, Polyacrylamide GelEscherichia coliExteinsGene ExpressionGenetic VectorsHistidineHydrogen-Ion ConcentrationMaltose-Binding ProteinsNitrogen IsotopesAspartic AcidCarbon IsotopesCysteineHistidineMaltose-Binding ProteinsNitrogen IsotopesRecombinant Fusion ProteinsInteinNMR spectroscopyProtein overexpressionProtein splicingSDS-PAGE

Identifiers

PMID32144663
PMCPMC7325523
OpenAlexW3009764577

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.