ArticlePathogens (Basel, Switzerland)2020
Development of a High-Throughput Serum Neutralization Test Using Recombinant Pestiviruses Possessing a Small Reporter Tag.
Article in Pathogens (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Rapid Quantification of Bluetongue Virus-Neutralizing Antibodies Using Bioluminescent Reporter-Expressing Viruses.Vaccines · 2025Article
- A nanoluciferase-tagged Schmallenberg virus (SBV): an efficient tool for measuring and tracking viral infection dynamics.Journal of medical microbiology · 2025Article
- Article
- Direct TaqMan assay for the detection and genotyping of bovine viral diarrhea virus types 1 and 2.Archives of virology · 2024Article
- Assessment of the Safety Profile of Chimeric Marker Vaccine against Classical Swine Fever: Reversion to Virulence Study.Viruses · 2024Article
- Establishment of a superinfection exclusion method for pestivirus titration using a recombinant reporter pestiviruses.The Journal of veterinary medical science · 2024Article
- Development of a dual immunochromatographic test strip to detect E2 and EFrontiers in microbiology · 2024Article
- Article
- Characterization of host factors associated with the internal ribosomal entry sites of foot-and-mouth disease and classical swine fever viruses.Scientific reports · 2022Article
- Characteristics of Classical Swine Fever Virus Variants Derived from Live Attenuated GPEViruses · 2021Article
- Characterization of a Cytopathogenic Reporter CSFV.Viruses · 2021Article
- Article
- Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications.International journal of molecular sciences · 2020Review
- Classical Swine Fever: A Truly Classical Swine Disease.Pathogens (Basel, Switzerland) · 2020Article
- Recent Advances in the Diagnosis of Classical Swine Fever and Future Perspectives.Pathogens (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
A serum neutralization test (SNT) is an essential method for the serological diagnosis of pestivirus infections, including classical swine fever, because of the cross reactivity of antibodies against pestiviruses and the non-quantitative properties of antibodies in an enzyme-linked immunosorbent assay. In conventional SNTs, an immunoperoxidase assay or observation of cytopathic effect after incubation for 3 to 7 days is needed to determine the SNT titer, which requires labor-intensive or time-consuming procedures. Therefore, a new SNT, based on the luciferase system and using classical swine fever virus, bovine viral diarrhea virus, and border disease virus possessing the 11-amino-acid subunit derived from NanoLuc luciferase was developed and evaluated; this approach enabled the rapid and easy determination of the SNT titer using a luminometer. In the new method, SNT titers can be determined tentatively at 2 days post-infection (dpi) and are comparable to those obtained by conventional SNTs at 3 or 4 dpi. In conclusion, the luciferase-based SNT can replace conventional SNTs as a high-throughput antibody test for pestivirus infections.
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