Evidence map›Paper›PMID 32143180›Full record

ArticleEBioMedicine2020

Bone marrow mesenchymal stem cells combined with ultra-purified alginate gel as a regenerative therapeutic strategy after discectomy for degenerated intervertebral discs.

Daisuke Ukeba, Hideki Sudo, Takeru Tsujimoto, Katsuro Ura, Katsuhisa Yamada, Norimasa Iwasaki

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EBioMedicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
4.3field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 69 citations in OpenAlex.

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  11. Tissue engineering strategies for treating intervertebral disc degeneration.Frontiers in bioengineering and biotechnology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Daisuke UkebaFaculty of Medicine and Graduate of Medicine, Department of Orthopedic Surgery, Hokkaido University, Sapporo, Hokkaido, Japan.
Hideki SudoFaculty of Medicine and Graduate of Medicine, Department of Orthopedic Surgery, Hokkaido University, Sapporo, Hokkaido, Japan; Faculty of Medicine and Graduate of Medicine, Department of Advanced Medicine for Spine and Spinal Cord Disorders, Hokkaido University, Sapporo, Hokkaido, Japan. Electronic address: hidekisudo@yahoo.co.jp.
Takeru TsujimotoFaculty of Medicine and Graduate of Medicine, Department of Orthopedic Surgery, Hokkaido University, Sapporo, Hokkaido, Japan.
Katsuro UraFaculty of Medicine and Graduate of Medicine, Department of Orthopedic Surgery, Hokkaido University, Sapporo, Hokkaido, Japan.
Katsuhisa YamadaFaculty of Medicine and Graduate of Medicine, Department of Orthopedic Surgery, Hokkaido University, Sapporo, Hokkaido, Japan; Faculty of Medicine and Graduate of Medicine, Department of Advanced Medicine for Spine and Spinal Cord Disorders, Hokkaido University, Sapporo, Hokkaido, Japan.
Norimasa IwasakiFaculty of Medicine and Graduate of Medicine, Department of Orthopedic Surgery, Hokkaido University, Sapporo, Hokkaido, Japan.
Hokkaido University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBecause the regenerative ability of intervertebral discs (IVDs) is restricted, defects caused by discectomy may induce insufficient tissue repair leading to further IVD degeneration. An acellular bioresorbable biomaterial based on ultra-purified alginate (UPAL) gel was developed to fill the IVD cavity and prevent IVD degeneration. However, an acellular matrix-based strategy may have limitations, particularly in the elderly population, who exhibit low self-repair capability. Therefore, further translational studies involving product combinations, such as UPAL gel plus bone marrow-derived mesenchymal stem cells (BMSCs), are required to evaluate the regenerative effects of BMSCs embedded in UPAL gel on degenerated IVDs.

methodsRabbit BMSCs and nucleus pulposus cells (NPCs) were co-cultured in a three-dimensional (3D) system in UPAL gel. In addition, rabbit or human BMSCs combined with UPAL gel were implanted into IVDs following partial discectomy in rabbits with degenerated IVDs.

findingsGene expression of NPC markers, growth factors, and extracellular matrix was significantly increased in the NPC and BMSC 3D co-culture compared to that in each 3D mono-culture. In vivo, whereas UPAL gel alone suppressed IVD degeneration as compared to discectomy, the combination of BMSCs and UPAL gel exerted a more potent effect to induce IVD regeneration. Similar IVD regeneration was observed using human BMSCs.

interpretationThese findings demonstrate the therapeutic potential of BMSCs combined with UPAL gel as a regenerative strategy following discectomy for degenerated IVDs.

fundingMinistry of Education, Culture, Sports, Science, and Technology of Japan, Japan Agency for Medical Research and Development, and the Mochida Pharmaceutical Co., Ltd.

Indexed as

RegenerationAlginatesAnimalsCells, CulturedDiskectomyHumansHydrogelsIntervertebral Disc DegenerationMaleMesenchymal Stem CellsMesenchymal Stem Cell TransplantationNucleus PulposusRabbitsAlginatesHydrogelsBiomaterialBone marrow mesenchymal stem cellHerniated discIntervertebral disc

Identifiers

PMID32143180
PMCPMC7057222
OpenAlexW3009081683

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.