ArticleNano letters2020
Fluctuation-Based Super-Resolution Traction Force Microscopy.
Article in Nano letters, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 70 citations in OpenAlex.
- Optical Detection of Cellular Signals at Material Interfaces.Advanced healthcare materials · 2026Review
- ONE microscopy.Nature protocols · 2026Review
- Traction Force Microscopy for Viscoelastic Substrates: A Semi-Analytical Method.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Myocardial mechanics displaying on visual structural color hydrogel microcolumn arrays.Journal of nanobiotechnology · 2026Article
- Traction Force Microscopy with DNA FluoroCubes.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Expansion and fluctuations-enhanced microscopy for nanoscale molecular profiling of cells and tissues.Nature protocols · 2026Review
- Understanding and measuring mechanical signals in the tumor stroma.FEBS open bio · 2025Review
- Aligned Collagen Fibers Drive Distinct Traction Force Signatures to Regulate Contact Guidance.ACS nano · 2025Article
- Analytical methods in studying cell force sensing: principles, current technologies and perspectives.Regenerative biomaterials · 2025Review
- Cell-cell communication: new insights and clinical implications.Signal transduction and targeted therapy · 2024Review
- 3D Traction Force Microscopy in Biological Gels: From Single Cells to Multicellular Spheroids.Annual review of biomedical engineering · 2024Review
- Molecular Force Sensors for Biological Application.International journal of molecular sciences · 2024Review
- Bayesian traction force estimation using cell boundary-dependent force priors.Biophysical journal · 2023Article
- High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation.Nature methods · 2023Article
- Fluorescence fluctuation-based super-resolution microscopy: Basic concepts for an easy start.Journal of microscopy · 2022Review
- Nanoscale Tracking Combined with Cell-Scale Microrheology Reveals Stepwise Increases in Force Generated by Cancer Cell Protrusions.Nano letters · 2022Article
- Wrinkle force microscopy: a machine learning based approach to predict cell mechanics from images.Communications biology · 2022Article
- Biophysical Approaches for Applying and Measuring Biological Forces.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Article
- Super-Resolution Radial Fluctuations (SRRF) Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Cellular mechanics play a crucial role in tissue homeostasis and are often misregulated in disease. Traction force microscopy is one of the key methods that has enabled researchers to study fundamental aspects of mechanobiology; however, traction force microscopy is limited by poor resolution. Here, we propose a simplified protocol and imaging strategy that enhances the output of traction force microscopy by increasing i) achievable bead density and ii) the accuracy of bead tracking. Our approach relies on super-resolution microscopy, enabled by fluorescence fluctuation analysis. Our pipeline can be used on spinning-disk confocal or widefield microscopes and is compatible with available analysis software. In addition, we demonstrate that our workflow can be used to gain biologically relevant information and is suitable for fast long-term live measurement of traction forces even in light-sensitive cells. Finally, using fluctuation-based traction force microscopy, we observe that filopodia align to the force field generated by focal adhesions.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.