ArticleCancer medicine2020
Reliable blood cancer cells' telomere length evaluation by qPCR.
Article in Cancer medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Lengthened telomere length in peripheral blood leukocytes in patients with rheumatoid arthritis complicated with airway disease.BMC rheumatology · 2025Article
- Telomere shortening in middle-aged and elderly individuals with varying severities of obstructive sleep apnea.Scientific reports · 2025Article
- Patchouli Ethanol ExtractAging medicine (Milton (N.S.W)) · 2025Article
- Transduction of Human Fetal Liver Hematopoietic CD34+ Stem and Progenitor Cells into a Cell Line by Enhancing Telomerase Activity.International journal of hematology-oncology and stem cell research · 2024Article
- T-cell lymphocytes' aging clock: telomeres, telomerase and aging.Biogerontology · 2024Review
- Shorter telomere length increases the risk of lymphocyte immunodeficiency: A Mendelian randomization study.Immunity, inflammation and disease · 2024Article
- EPAS1 prevents telomeric damage-induced senescence by enhancing transcription ofZoological research · 2023Article
- Spotlight onGenes · 2023Article
- Article
- Review
- Cross-species Association Between Telomere Length and Glucocorticoid Exposure.The Journal of clinical endocrinology and metabolism · 2021Article
- Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature.PloS one · 2021Article
- Selective Elimination of Osteosarcoma Cell Lines with Short Telomeres by Ataxia Telangiectasia and Rad3-Related Inhibitors.ACS pharmacology & translational science · 2020Article
- Reliable blood cancer cells' telomere length evaluation by qPCR.Cancer medicine · 2020Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTelomere shortening is linked to a range of different human diseases, hence reliable measurement methods are needed to uncover such associations. Among the plethora of telomere length measurement methods, qPCR is reported as easy to conduct and a cost-effective approach to study samples with low DNA amounts.
methodsCancer cells' telomere length was evaluated by relative and absolute qPCR methods.
resultsRobust and reproducible telomere length measurements were optimized taking into account a careful reference gene selection and by knowing the cancer cells ploidy. qPCR data were compared to "gold standard" measurement from terminal restriction fragment (TRF).
conclusionsOur study provides guidance and recommendations for accurate telomere length measurement by qPCR in cancer cells, taking advantage of our expertise in telomere homeostasis investigation in primary cutaneous T-cell lymphomas. Furthermore, our data emphasize the requirement of samples with both, high DNA quality and high tumor cells representation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.