Evidence map›Paper›PMID 32140882›Full record

ArticleBiotechnology letters2020

Development of a sensitive monoclonal antibody-based sandwich ELISA to detect Vip3Aa in genetically modified crops.

Weixiao Liu, Xuri Liu, Chao Liu, Zhe Zhang, Wujun Jin

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology letters, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 10 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Weixiao LiuBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. liuweixiao@caas.cn.
Xuri LiuDepartment of Food and Biological Engineering, Handan Polytechnic College, Handan, 056001, China.
Chao LiuState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Science, Beijing, 100101, China.
Zhe ZhangBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Wujun JinBiotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. jinwujun@caas.cn.
Biotechnology Research Institute · CNChinese Academy of Agricultural Sciences · CNHandan Polytechnic College · CNState Key Laboratory of Stem Cell and Reproductive Biology

Funding

Fundamental Research Funds for Non-profit Scientific Institution Grant no. 1610392018008National Transgenic Major Program of China No. 2017ZX08013001
6 · The paper itself

Abstract

objectivesTo develop a sensitive monoclonal antibody-based sandwich enzyme-linked immunosorbent assay (ELISA) to detect Vip3Aa in genetically modified (GM) crops and their products.

resultsVegetative insecticidal proteins (Vips) are secreted by Bacillus thuringiensis (Bt) and are known to be toxic to Lepidoptera species. Vip3Aa family proteins, Vip3Aa19 and Vip3Aa20, were successfully applied in GM crops to confer an effective and persistent insecticidal resistance. A sensitive monoclonal antibody-based sandwich ELISA was developed to detect Vip3Aa in GM crops and their products. Two monoclonal antibodies were raised against the overexpressed and purified His-Vip3Aa20, were purified from mouse ascites and characterized. A sandwich ELISA method was developed using the 2G3-1D7 monoclonal antibody for capture and the biotin-labeled 1F9-1F5 monoclonal antibody for detection of Vip3Aa20. The linear detection range of the method was found to be approximately 31.25-500 pg/ml, with a sensitivity of 10.24 pg/ml.

conclusionsThe established ELISA was effective for detecting Vip3Aa family proteins other than Vip3Aa8, and was successfully applied in the detection of Vip3Aa20 and Vip3Aa19 expressed in transgenic maize and cotton.

Indexed as

Bacterial ProteinsAnimalsAntibodies, MonoclonalCrops, AgriculturalEnzyme-Linked Immunosorbent AssayFood SafetyMaleMiceMice, Inbred BALB CPlants, Genetically ModifiedSensitivity and SpecificityAntibodies, MonoclonalBacterial ProteinsVip3A protein, Bacillus thuringiensisCottonELISAGenetically modified cropsMaizeMonoclonal antibodyVip3Aa

Identifiers

PMID32140882
PMCPMC7354279
OpenAlexW3009400990

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.