Evidence map›Paper›PMID 32140734›Full record

ArticleArchives of microbiology2020

Yeast engineered translucent cell wall to provide its endosymbiont cyanobacteria with light.

Hoda Ebrahimi, Farideh Siavoshi, Samira Heydari, Abdolfattah Sarrafnejad, Parastoo Saniee

Abstract read
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In one paragraph

Article in Archives of microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 62% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Hoda EbrahimiDepartment of Microbiology, School of Biology, University College of Sciences, University of Tehran, Enghelab Avenue, 14176-14411, Tehran, Iran.
Farideh SiavoshiDepartment of Microbiology, School of Biology, University College of Sciences, University of Tehran, Enghelab Avenue, 14176-14411, Tehran, Iran. farideh.siavoshi@yahoo.com.
Samira HeydariDepartment of Microbiology, School of Biology, University College of Sciences, University of Tehran, Enghelab Avenue, 14176-14411, Tehran, Iran.
Abdolfattah SarrafnejadDepartment of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Parastoo SanieeDepartment of Microbiology and Microbial Biotechnology, Faculty of Life Science and Biotechnology, Shahid Beheshti University G.C., Tehran, Iran.
University of Tehran · IRShahid Beheshti University · IRTehran University of Medical Sciences · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, relationship between translucent property of yeast cell wall and occurrence of cyanobacteria inside the yeast vacuole was examined. Microscopic observations on fruit yeast Candida tropicalis showed occurrence of bacterium-like bodies inside the yeast vacuole. Appearance of vacuoles as distinct cavities indicated the perfect harvesting of light by the yeast's cell wall. Transmission electron microscopy observation showed electron-dense outer and electron-lucent inner layers in yeast cell wall. Cyanobacteria-specific 16S rRNA gene was amplified from total DNA of yeast. Cultivation of yeast in distilled water led to excision of intracellular bacteria which grew on cyanobacteria-specific medium. Examination of wet mount and Gram-stained preparations of excised bacteria showed typical bead-like trichomes. Amplification of cyanobacteria-specific genes, 16S rRNA, cnfR and dxcf, confirmed bacterial identity as Leptolyngbya boryana. These results showed that translucent cell wall of yeast has been engineered through evolution for receiving light for vital activities of cyanobacteria.

Indexed as

SymbiosisCandida tropicalisCell WallCyanobacteriaGenes, BacterialMicroscopy, Electron, TransmissionRNA, Ribosomal, 16SVacuolesRNA, Ribosomal, 16SCell wallEndosymbiotic cyanobacteriaTranslucentYeast

Identifiers

PMID32140734
OpenAlexW3009344744

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.