ArticleHeliyon2020
A colorimetric bioassay for quantitation of both basal and insulin-induced glucose consumption in 3T3-L1 adipose cells.
Article in Heliyon, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 13 citations in OpenAlex.
- DHA ModulatesInternational journal of molecular sciences · 2025Article
- Supernatants from Newly IsolatedBiomedicines · 2024Article
- DHA-Provoked Reduction in Adipogenesis and Glucose Uptake Could Be Mediated by Gps2 Upregulation in Immature 3T3-L1 Cells.International journal of molecular sciences · 2023Article
- Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems.Biosensors · 2023Article
- Preparation of Cell-Loaded Microbeads as Stable and Injectable Delivery Platforms for Tissue Engineering.Biomimetics (Basel, Switzerland) · 2023Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionThe quantitation of glucose consumption in animal cell cultures is mainly based on the use of radiolabeled or fluorescent analogues, resulting in expensive and tedious procedures, requiring special equipment and, sometimes, with potential health and environmental risks.
objectivesThe objective of this work was to evaluate the application of a blood plasma colorimetric assay to quantify glucose consumption in
methodsWe worked with 3T3-L1 adipose cells differentiated by 7-8 days, which were exposed to different initial glucose concentrations (5.5, 2.8 and 1.4 mM) for variable times, either in the absence or the presence of 100 nM insulin. Using a commercial colorimetric glucose assay, extracellular glucose was determined, and glucose uptake was calculated as the difference between the initial and final glucose concentration.
resultsThe colorimetric assay allowed us to quantify glucose uptake in our cell model, observing a linear response over time (
conclusionsA simple and low-cost bioassay is proposed to quantify glucose consumption in 3T3-L1 adipose cells.
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Registered trials
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