Evidence map›Paper›PMID 32140075›Full record

ArticleInternational journal of biological sciences2020

ROBO4 deletion ameliorates PAF-mediated skin inflammation via regulating the mRNA translation efficiency of LPCAT1/LPCAT2 and the expression of PAF receptor.

Xiaoqiang Xiao, Xi Zhuang, Ciyan Xu, Haoyu Chen, Weiquan Zhu, ChiPui Pang, Mingzhi Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of biological sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Xiaoqiang XiaoJoint Shantou International Eye Center, Shantou University and the Chinese University of Hong Kong, Shantou, China.
Xi ZhuangJoint Shantou International Eye Center, Shantou University and the Chinese University of Hong Kong, Shantou, China.
Ciyan XuJoint Shantou International Eye Center, Shantou University and the Chinese University of Hong Kong, Shantou, China.
Haoyu ChenJoint Shantou International Eye Center, Shantou University and the Chinese University of Hong Kong, Shantou, China.
Weiquan ZhuDepartment of Medicine, Program in Molecular Medicine, University of Utah.
ChiPui PangJoint Shantou International Eye Center, Shantou University and the Chinese University of Hong Kong, Shantou, China.
Mingzhi ZhangJoint Shantou International Eye Center, Shantou University and the Chinese University of Hong Kong, Shantou, China.
Chinese University of Hong Kong · CNShantou University · CNUniversity of Utah · US

Funding

Targeting ARF6, A Convergence Point For Multiple Pathways of Diabetic RetinopathyR01EY025342 · NEI · UNIVERSITY OF UTAH · PI ZHU, WEIQUAN (WENDY) · 2015 to 2019
$1.7M
NEI NIH HHS R01 EY025342
6 · The paper itself

Abstract

The diminished level of platelet-activating factor acetylhydrolase (PAFAH) in milk causes an enhanced level of platelet activating factor (PAF) in the skin, leading to a severe hair loss phenotype during neonatal pup's lactation. The deletion of very-low-density-lipoprotein receptor (VLDLR) prevents the expression and secretion of PAFAH. Here we revealed that deletion of Roundabout 4 (ROBO4) in mice ameliorated hair loss phenotype via reducing PAF concentration in skin. As a consequence, the neonatal pups with ROBO4 deletion lactated by mother with VLDLR deletion showed normal hair phenotype during lactation. In details,ROBO4 deletion reduced the protein but not mRNA expression of two PAF synthetic enzymes LPCAT1/LPCAT2 in macrophage as well as the expression of PAF receptor in both macrophage and ocular tissue, but increased PAFAH protein in serum. On the other hand, RNA expression profile analysis in macrophages revealed that the genes involving in oxidative phosphorylation and ribosome obviously decreased their expression in response to ROBO4 deletion. Moreover, through High Performance Liquid Chromatography (HPLC) analysis, we found that ATP concentration also reduced in ROBO4 deletion macrophages. Because ribosome and energy are very important factors for the mRNA translation, we then tested whether ROBO4 deletion affects LPCAT1/LPCAT2 mRNA translation using polyribosome assay. As expected, the mRNA level of LPCAT1/LPCAT2 significantly decreased in polyribosome in ROBO4 deletion macrophage comparing to that of wild type. Additionally, mice with ROBO4 deletion suppressed LPS-induced IL-6 expression as well as the phosphorylation of p44/42 and p65, but enhanced the AKT phosphorylation. Collectively, ROBO4 deletion alleviates PAF- and LPS-mediated inflammation. And above results also indicate PAF signal might be a crosstalk point of ROBO4- and VLDLR-activated pathways.

Indexed as

1-Acylglycerophosphocholine O-AcyltransferaseAnimalsBlotting, WesternComputational BiologyEnzyme-Linked Immunosorbent AssayInflammationMass SpectrometryMiceMice, Inbred C57BLPlatelet Activating FactorPlatelet Membrane GlycoproteinsProtein BiosynthesisReceptors, Cell SurfaceReceptors, G-Protein-CoupledRNA, MessengerSequence Analysis, RNA1-Acylglycerophosphocholine O-AcyltransferaseLpcat1 protein, mouseLPCAT2 protein, mousePlatelet Activating Factorplatelet activating factor receptorPlatelet Membrane GlycoproteinsReceptors, Cell SurfaceReceptors, G-Protein-CoupledRNA, MessengerRobo4 protein, mousehair lossoxidative phosphorylationPAFribosomeROBO4

Identifiers

PMID32140075
PMCPMC7053341
OpenAlexW3005875457

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.