ArticleScientific reports2020
Rapid isothermal duplex real-time recombinase polymerase amplification (RPA) assay for the diagnosis of equine piroplasmosis.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Equine alveolar echinococcosis: a focus on epidemiology, clinical and pathological features, and diagnostics.Journal of equine science · 2026Review
- Isothermal nucleic acid amplification techniques: A comprehensive overview on their principle & applications as alternative to PCR.Practical laboratory medicine · 2026Review
- Development of a novel duplex recombinase polymerase amplification (DRPA) assay for rapid detection of carbapenem-resistant Acinetobacter baumannii.BMC infectious diseases · 2026Article
- Research progress on diagnostic techniques for differentFrontiers in cellular and infection microbiology · 2025Review
- Development and Application of a Duplex RT-RPA Assay for the Simultaneous Detection ofViruses · 2024Article
- Development of a multiplex RT-RPA assay for simultaneous detection of three viruses in cucurbits.Molecular plant pathology · 2023Article
- Rapid, sensitive, and specific, low-resource molecular detection of Hendra virus.One health (Amsterdam, Netherlands) · 2023Article
- Rapid detection of alveolar echinococcosis in hepatic nodules of horses by recombinase polymerase amplification assay.Veterinary and animal science · 2023Article
- Review
- Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications.Frontiers in cellular and infection microbiology · 2022Review
- Development of Nested PCR and Duplex Real-Time Fluorescence Quantitative PCR Assay for the Simultaneous Detection ofFrontiers in veterinary science · 2022Article
- Rapid, Cheap, and Effective COVID-19 Diagnostics for Africa.Diagnostics (Basel, Switzerland) · 2021Review
- CRISPR Tackles Emerging Viral Pathogens.Viruses · 2021Review
- A recombinase polymerase amplification-based assay for rapid detection of Chlamydia psittaci.Poultry science · 2021Article
- Challenges in Tick-Borne Pathogen Detection: The Case forPathogens (Basel, Switzerland) · 2021Review
- Development of a Recombinase Polymerase Amplification Assay for Schistosomiasis Japonica Diagnosis in the Experimental Mice and Domestic Goats.Frontiers in cellular and infection microbiology · 2021Article
- Development of a recombinase polymerase amplification assay for rapid detection of Haemophilus parasuis in tissue samples.Veterinary medicine and science · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Equine piroplasmosis (EP) is a severe disease of horses caused by the tick-borne protozoa Theileria equi (T. equi) and Babesia caballi (B. caballi). Infectious carriers are not always symptomatic, meaning there is a risk to non-enzootic areas. Regulatory tests for EP include sero-epidemiological methods for equine babesiosis, but these lack specificity due to cross-reactivity with other Babesia species. In this study, we present a real-time quantitative recombinase polymerase amplification (qRPA) method for fast simultaneous detection of both T. equi and B. caballi. In this method, primers and probes targeting the 18S rRNA gene of both T. equi and B. caballi, the ema-1 gene of T. equi and the bc48 gene of B. caballi were designed and evaluated. The sensitivity of qRPA was evaluated using the pUC57 plasmid DNA containing the target gene. For the pUC57-bc48 gene DNA, the R
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.