Evidence map›Paper›PMID 32139598›Full record

ReviewJournal of cell science2020

Imaging of the immune system - towards a subcellular and molecular understanding.

Lai Wen, Zhichao Fan, Zbigniew Mikulski, Klaus Ley

Open access · bronzeAbstract readReview
In one paragraph

Review in Journal of cell science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
  2. Review
  3. Visualisation of Host-Pathogen Communication.Advances in experimental medicine and biology · 2023
    Article
  4. Review
  5. [Research progress on the fluorescence resonance energy transfer-based polymer micelles as drug carriers].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2022
    Review
  6. Article
  7. A humanized βCell reports · 2022
    Article
  8. Article
  9. Review
  10. Article
  11. Visualization of integrin molecules by fluorescence imaging and techniques.Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Lai WenLaboratory of Inflammation Biology, La Jolla Institute for Immunology, 9420 Athena Circle Drive, La Jolla, CA 92037, USA.ORCID 0000-0001-9760-3403
Zhichao FanDepartment of Immunology, School of Medicine, UConn Health, 263 Farmington Avenue, Farmington, CT 06030, USA.ORCID 0000-0001-5385-9626
Zbigniew MikulskiMicroscopy Core Facility, La Jolla Institute for Immunology, 9420 Athena Circle Drive, La Jolla, CA 92037, USA.
Klaus LeyLaboratory of Inflammation Biology, La Jolla Institute for Immunology, 9420 Athena Circle Drive, La Jolla, CA 92037, USA klaus@lji.org.ORCID 0000-0001-9339-3672
La Jolla Institute for Immunology · USUConn Health · US

Funding

The Interaction of TPO and Integrins in Hematopoiesis CellsP01HL078784 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GINSBERG, MARK HOWARD · 2005 to 2019
$28.3M
Molecular mechanism and preclinical translation of beta2 integrin auto-inhibition on neutrophil arrest and inflammationR01HL145454 · NHLBI · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI FAN, ZHICHAO · 2019 to 2023
$2.1M
American Heart Association-American Stroke Association 18CDA34110426NHLBI NIH HHS P01 HL078784NHLBI NIH HHS R01 HL145454
6 · The paper itself

Abstract

Immune responses involve many types of leukocytes that traffic to the site of injury, recognize the insult and respond appropriately. Imaging of the immune system involves a set of methods and analytical tools that are used to visualize immune responses at the cellular and molecular level as they occur in real time. We will review recent and emerging technological advances in optical imaging, and their application to understanding the molecular and cellular responses of neutrophils, macrophages and lymphocytes. Optical live-cell imaging provides deep mechanistic insights at the molecular, cellular, tissue and organism levels. Live-cell imaging can capture quantitative information in real time at subcellular resolution with minimal phototoxicity and repeatedly in the same living cells or in accessible tissues of the living organism. Advanced FRET probes allow tracking signaling events in live cells. Light-sheet microscopy allows for deeper tissue penetration in optically clear samples, enriching our understanding of the higher-level organization of the immune response. Super-resolution microscopy offers insights into compartmentalized signaling at a resolution beyond the diffraction limit, approaching single-molecule resolution. This Review provides a current perspective on live-cell imaging

Indexed as

MacrophagesMicroscopyFRETImagingImmune cellsIntravital imagingMicroscopyMolecular imagingSubcellular imaging

Identifiers

PMID32139598
PMCPMC7075077
OpenAlexW3010438223

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.