ArticleBMC biotechnology2020
An efficient gene disruption method for the woody plant pathogen Botryosphaeria dothidea.
Article in BMC biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Functional genome analysis reveals that serine carboxypeptidaseFrontiers in plant science · 2025Article
- MdBT2 regulates nitrogen-mediated cuticular wax biosynthesis via a MdMYB106-MdCER2L1 signalling pathway in apple.Nature plants · 2024Article
- Metabolome and Transcriptome Profiling Reveals the Function of MdSYP121 in the Apple Response toInternational journal of molecular sciences · 2023Article
- LysM protein BdLM1 ofFrontiers in plant science · 2023Article
- A Secreted Lignin Peroxidase Required for Fungal Growth and Virulence and Related to Plant Immune Response.International journal of molecular sciences · 2022Article
- Endophytic bacteriumFrontiers in microbiology · 2022Article
- The heat shock protein 20 gene editing suppresses mycelial growth ofFrontiers in microbiology · 2022Article
- The Cutinase Bdo_10846 Play an Important Role in the Virulence ofInternational journal of molecular sciences · 2021Article
- Candidate Effectors fromInternational journal of molecular sciences · 2021Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBotryosphaeria dothidea causes apple white rot and infects many tree plants. Genome data for B. dothidea are available and many pathogenesis-related genes have been predicted. However, a gene manipulation method is needed to study the pathogenic mechanism of B. dothidea.
resultsWe established a gene disruption (GD) method based on gene homologous recombination (GHR) for B. dothidea using polyethylene glycol-mediated protoplast transformation. The results showed that a GHR cassette gave much higher GD efficiency than a GHR plasmid. A high GD efficiency (1.3 ± 0.14 per 10
conclusionThe established GD method is efficient and convenient and has potential for studying gene functions and the pathogenic mechanisms of B. dothidea and other coenocytic fungi.
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