ArticleBMC plant biology2020
Transcriptome co-expression network analysis identifies key genes and regulators of ripening kiwifruit ester biosynthesis.
Article in BMC plant biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 57 citations in OpenAlex.
- OfWRKY48 coordinates floral aroma biosynthesis by dual regulation of MEP pathway and carotenoid metabolism inHorticulture research · 2026Article
- Evolution of metabolite and volatile compounds in Chinese bayberry during juice processing, fermentation, and distillation.Food chemistry: X · 2026Article
- Characterization of Volatile Profile of Different Kiwifruits (Foods (Basel, Switzerland) · 2026Article
- Advances in Kiwifruit Postharvest Management: Convergence of Physiological Insights, Omics, and Nondestructive Technologies.Current issues in molecular biology · 2025Review
- Genome-Wide Identification and Expression Profiling of the Invertase Genes Involved in Sugar Metabolism and Accumulation inInternational journal of molecular sciences · 2025Article
- Volatile aromatic substances analysis of different temperature stored 'Docteur Jules Guyot' pear (Frontiers in plant science · 2025Article
- Article
- Analysis of Aroma Characteristics of 'Binzi' and 'Xiangguo' Apple-Ancient Cultivars in China.Foods (Basel, Switzerland) · 2024Article
- Kiwifruit in the Omics Age: Advances in Genomics, Breeding, and Beyond.Plants (Basel, Switzerland) · 2024Review
- Mechanism of abscisic acid in promoting softening of postharvest 'Docteur Jules Guyot' pear (Frontiers in plant science · 2024Article
- Insights into the aroma volatiles and the changes of expression of ester biosynthesis candidate genes during postharvest storage of European pear.Frontiers in plant science · 2024Article
- Transcriptome Co-Expression Network Analysis of Peach Fruit with Different Sugar Concentrations Reveals Key Regulators in Sugar Metabolism Involved in Cold Tolerance.Foods (Basel, Switzerland) · 2023Article
- Identification and Characterization of Long Non-Coding RNAs: Implicating Insights into Their Regulatory Role in Kiwifruit Ripening and Softening during Low-Temperature Storage.Plants (Basel, Switzerland) · 2023Article
- Identification and characterization ofFrontiers in plant science · 2023Article
- Characterization of key aroma compounds and regulation mechanism of aroma formation in local Binzi (Malus pumila × Malus asiatica) fruit.BMC plant biology · 2022Article
- Functional diversity and metabolic engineering of plant-specialized metabolites.Life metabolism · 2022Review
- Alcohol acyl transferase genes at a high-flavor intensity locus contribute to ester biosynthesis in kiwifruit.Plant physiology · 2022Article
- Consensus co-expression network analysis identifies AdZAT5 regulating pectin degradation in ripening kiwifruit.Journal of advanced research · 2022Article
- Integration of Transcriptome and Metabolome Provides New Insights to Flavonoids Biosynthesis inFrontiers in plant science · 2022Article
- The Effect of 1-MCP on the Expression of Carotenoid, Chlorophyll Degradation, and Ethylene Response Factors in 'Qihong' Kiwifruit.Foods (Basel, Switzerland) · 2021Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundAroma is an important organoleptic quality for fruit and has a large influence on consumer preference. Kiwifruit esters undergo rapid and substantial changes contributing to the flavor during fruit ripening. Part of enzymes and their coding genes have been indicated potential candidates for flavor-related esters synthesis. However, there still exist obvious gaps in the biosynthetic pathways of esters and the mechanisms regulating ester biosynthesis in kiwifruit remain unknown.
resultsUsing gas chromatography-mass spectrometry (GC-MS), volatile compounds of kiwifruit were quantified in response to ethylene (ETH, 100 μl/l, 24 h, 20 °C) and 1-methylcyclopropene (1-MCP, 1 μl/l, 24 h, 20 °C). The results indicated that esters showed the most substantial changes enhanced by ethylene and were inhibited by 1-MCP. Correlations between RNA-seq results and concentrations of esters, constructed using Weighted Gene Co-Expression Network Analysis (WGCNA) indicated that three structural genes (fatty acid desaturase, AdFAD1; aldehyde dehydrogenase, AdALDH2; alcohol acyltransferase, AdAT17) had similar expression patterns that paralled the changes in total ester content, and AdFAD1 transcripts exhibited the highest correlation. In order to search for potential regulators for ester biosynthesis, 14 previously reported ethylene-responsive transcription factors (TFs) were included in the correlation analysis with esters and their biosynthetic genes. Using dual-luciferase assay, the in vivo regulatory activities of TFs on ester biosynthetic gene promoters were investigated and the results indicated that AdNAC5 and AdDof4 (DNA binding with one finger) trans-activated and trans-suppressed the AdFAD1 promoter.
conclusionsThe present study advanced the molecular basis of ripening-related ester biosynthesis in kiwifruit by identifying three biosynthetic related genes AdFAD1, AdALDH2 and AdAT17 by transcriptome analysis, and highlighted the function of two TFs by transactivation studies.
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