ArticleMethods and protocols2020
A Rapid and High Throughput MIC Determination Method to Screen Uranium Resistant Microorganisms.
Article in Methods and protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
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- Surfactin-like lipopeptides contribute to interference competition of Bacillus velezensis against the marine Flavobacteriaceae in phycosphere.Environmental microbiome · 2026Article
- Isolation and molecular-proteomic characterization of metallothionein-producing heavy metal-resistant bacteria for industrial wastewater bioremediation.Antonie van Leeuwenhoek · 2026Article
- Characterization of Bacillus atrophaeus Strain from the Composite Material Surface in Arctic Conditions and Its Sensitivity to CuO Nanoparticles.Current microbiology · 2026Article
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- Whole-genome analysis of Lysinibacillus boronitolerans MSR1: A dairy-isolated multidrug-resistant and non-pathogenic strain.PloS one · 2025Article
- Multimodal cadmium resistance and its regulatory networking in Pseudomonas aeruginosa strain CD3.Scientific reports · 2024Article
- Unlocking the therapeutic potential of Nigella sativa extract: phytochemical analysis and revealing antimicrobial and antioxidant marvels.BMC complementary medicine and therapies · 2024Article
- Evaluation of multi-heavy metal tolerance traits of soil-borne fungi for simultaneous removal of hazardous metals.World journal of microbiology & biotechnology · 2024Article
- Antimicrobial resistance level and conjugation permissiveness shape plasmid distribution in clinical enterobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
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- Genome-Wide Analysis of Innate Susceptibility Mechanisms ofAntibiotics (Basel, Switzerland) · 2022Article
- Degradation of Uranium-Contaminated Decontamination Film by UV Irradiation and Microbial Biodegradation.Microbial ecology · 2022Article
- Bioaccumulation and detoxification of trivalent arsenic by Achromobacter xylosoxidans BHW-15 and electrochemical detection of its transformation efficiency.Scientific reports · 2021Article
- Announcing the Availability of a Culture Collection of Uranium-Resistant Microbial Assemblages (CURMA) Obtained from Metalliferous Soils of the Savannah River Site, USA.Microbiology resource announcements · 2020Article
- Is Long-Term Heavy Metal Exposure Driving Carriage of Antibiotic Resistance in Environmental Opportunistic Pathogens: A Comprehensive Phenomic and Genomic Assessment UsingFrontiers in microbiology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The assessment of minimum inhibitory concentration (MIC) is a conventional technique used for the screening of microbial resistance against antibiotics, biocides, and contaminants such as heavy metals. However, as part of our ongoing work, we have observed biases associated with using traditional liquid MIC method to screen microbial heavy metal resistance, including both bacterial and fungal strains. Specifically, the addition of uranium into synthetic media causes immediate precipitation prior to the initiation of microbial growth, thus hampering the optical density measurements, and the obtained MIC values are thus flawed and inaccurate. To address this discrepancy, we report the optimization and development of a serial-dilution-based MIC method conducted on solid growth media supplemented with uranium, which is more accurate, relative to the testing of MICs performed in liquid cultures. Notably, we report on the efficacy of this method to screen not only bacteria that are resistant to uranium but also demonstrate the successful application to yeast and fungal isolates, for their ability to resist uranium, is more accurate and sensitive relative to the liquid method. We believe that this newly developed method to screen heavy metal resistance, such as uranium, is far superior to the existing liquid MIC method and propose replacing the liquid assay with the solid plate MIC reported herein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.