ArticleFrontiers in chemistry2020
A PEGDA/DNA Hybrid Hydrogel for Cell-Free Protein Synthesis.
Article in Frontiers in chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 46 citations in OpenAlex.
- Applications of DNA Hydrogels in Osteoporotic Bone Defects.Journal of functional biomaterials · 2026Review
- Design and Characterization of DX-Tile DNA Nanostar-Based Hydrogels.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Comprehensive Review on DNA Hydrogels and DNA Origami-Enabled Wearable and Implantable Biosensors.Biosensors · 2025Review
- Application of biomimetic approaches in the treatment of neurological disorders.Materials today. Bio · 2025Review
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- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Architectural engineering of Cyborg Bacteria with intracellular hydrogel.Materials today. Bio · 2024Article
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- Ester Bonds for Modulation of the Mechanical Properties of Protein Hydrogels.International journal of molecular sciences · 2023Article
- Hydrogel-Based Multi-enzymatic System for Biosynthesis.Advances in biochemical engineering/biotechnology · 2023Review
- Solid-Phase Cell-Free Protein Synthesis and Its Applications in Biotechnology.Advances in biochemical engineering/biotechnology · 2023Article
- Hydrogels as functional components in artificial cell systems.Nature reviews. Chemistry · 2022Review
- Cation-Responsive and Photocleavable Hydrogels from Noncanonical Amphiphilic DNA Nanostructures.Nano letters · 2022Article
- Formulation of DNA Nanocomposites: Towards Functional Materials for Protein Expression.Polymers · 2021Article
- Development, Preparation, and Biomedical Applications of DNA-Based Hydrogels.Frontiers in bioengineering and biotechnology · 2021Review
- Silk fibroin as an additive for cell-free protein synthesis.Synthetic and systems biotechnology · 2020Article
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Authors and funding
9 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell-free protein synthesis (CFPS) has the advantage of rapid expression of proteins and has been widely implemented in synthetic biology and protein engineering. However, the critical problem limiting CFPS industrial application is its relatively high cost, which partly attributes to the overexpense of single-use DNA templates. Hydrogels provide a possible solution because they can preserve and reutilize the DNA templates in CFPS and have great potential in elevating the protein production yield of the CFPS. Here, we presented a low-cost hybrid hydrogel simply prepared with polyethylene glycol diacrylate (PEGDA) and DNA, which is capable of high-efficient and repeated protein synthesis in CFPS. Parameters governing protein production specific to hybrid hydrogels were optimized. Structures and physical properties of the hybrid hydrogel were characterized. Transcription and expression kinetics of solution phase system and gel phased systems were investigated. The results showed that PEGDA/DNA hydrogel can enhance the protein expression of the CFPS system and enable a repeated protein production for tens of times. This PEGDA/DNA hybrid hydrogel can serve as a recyclable gene carrier for either batch or continuous protein expression, and paves a path toward more powerful, scalable protein production and cell-free synthetic biology.
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