ArticleBiomedical optics express2020
Multiview microscopy of single cells through microstructure-based indirect optical manipulation.
Article in Biomedical optics express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed.
- Optically Controlled Dissolution Kinetics of Vaterite Microcapsules: Toward Novel Crystal Growth Strategies.Crystal growth & design · 2023Article
- Multimodal Optothermal Manipulations along Various Surfaces.ACS nano · 2023Article
- Optothermal rotation of micro-/nano-objects.Chemical communications (Cambridge, England) · 2023Review
- Article
- Universal optothermal micro/nanoscale rotors.Science advances · 2022Article
- Rapid computational cell-rotation around arbitrary axes in 3D with multi-core fiber.Biomedical optics express · 2021Article
- A virtual reality interface for the immersive manipulation of live microscopic systems.Scientific reports · 2021Article
- Visually precise, low-damage, single-cell spatial manipulation with single-pixel resolution.Chemical science · 2021Article
- Single-Cell Elasticity Measurement with an Optically Actuated Microrobot.Micromachines · 2020Article
- Pitch-rotational manipulation of single cells and particles using single-beam thermo-optical tweezers.Biomedical optics express · 2020Article
- Applications of Optically Controlled Gold Nanostructures in Biomedical Engineering.Frontiers in bioengineering and biotechnology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescent observation of cells generally suffers from the limited axial resolution due to the elongated point spread function of the microscope optics. Consequently, three-dimensional imaging results in axial resolution that is several times worse than the transversal. The optical solutions to this problem usually require complicated optics and extreme spatial stability. A straightforward way to eliminate anisotropic resolution is to fuse images recorded from multiple viewing directions achieved mostly by the mechanical rotation of the entire sample. In the presented approach, multiview imaging of single cells is implemented by rotating them around an axis perpendicular to the optical axis by means of holographic optical tweezers. For this, the cells are indirectly trapped and manipulated with special microtools made with two-photon polymerization. The cell is firmly attached to the microtool and is precisely manipulated with 6 degrees of freedom. The total control over the cells' position allows for its multiview fluorescence imaging from arbitrarily selected directions. The image stacks obtained this way are combined into one 3D image array with a multiview image processing pipeline resulting in isotropic optical resolution that approaches the lateral diffraction limit. The presented tool and manipulation scheme can be readily applied in various microscope platforms.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.