ArticleBiomedical optics express2020
Tissue imaging depth limit of stimulated Raman scattering microscopy.
Article in Biomedical optics express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Harnessing fluorescent probes for molecular diagnosis and theranostics of atherosclerosis.Drug delivery · 2026Review
- Visualizing Metabolism: Lipid-Based Raman Nanoreporters as an Enabling Platform for Metabolic Imaging and Precision Medicine.ACS nano medicine · 2026Review
- High-Throughput Volumetric Mapping Facilitated by Active Tissue SHRINK.Small methods · 2026Article
- Normalized Raman Imaging for Studies of Tissue Physiology of the Kidney.bioRxiv : the preprint server for biology · 2025Article
- Self-stacked small molecules for ultrasensitive, substrate-free Raman imaging in vivo.Nature biotechnology · 2025Article
- Photon-counting Raman spectroscopy at a MHz spectral rate for biochemical imaging of an entire organism.Nature communications · 2025Article
- Lipid Metabolic Heterogeneity during Early Embryogenesis Revealed by Hyper-3D Stimulated Raman Imaging.Chemical & biomedical imaging · 2025Article
- Assessing Drug Uptake and Response Differences in 2D and 3D Cellular Environments Using Stimulated Raman Scattering Microscopy.Analytical chemistry · 2024Article
- Investigation of commercial cut-resistant gloves claiming graphene additive content.Graphene and 2D materials · 2024Article
- Innovative Approaches for Drug Discovery: Quantifying Drug Distribution and Response with Raman Imaging.Analytical chemistry · 2024Review
- Measuring Drug Response with Single-Cell Growth Rate Quantification.Analytical chemistry · 2023Article
- Quantifying Inflammatory Response and Drug-Aided Resolution in an Atopic Dermatitis Model with Deep Learning.The Journal of investigative dermatology · 2023Article
- The sound of blood: photoacoustic imaging in blood analysis.Medicine in novel technology and devices · 2023Article
- Label-Free Cytometric Evaluation of Mitosis via Stimulated Raman Scattering Microscopy and Spectral Phasor Analysis.Analytical chemistry · 2023Article
- Biomolecular histology as a novel proxy for ancient DNA and protein sequence preservation.Ecology and evolution · 2022Article
- Tilt-angle stimulated Raman projection tomography.Optics express · 2022Article
- Bringing Vibrational Imaging to Chemical Biology with Molecular Probes.ACS chemical biology · 2022Article
- Quantitative Stimulated Raman Scattering Microscopy: Promises and Pitfalls.Annual review of analytical chemistry (Palo Alto, Calif.) · 2022Review
- Protein and lipid mass concentration measurement in tissues by stimulated Raman scattering microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- In vivo simultaneous nonlinear absorption Raman and fluorescence (SNARF) imaging of mouse brain cortical structures.Communications biology · 2022Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Stimulated Raman scattering (SRS) microscopy is a promising technique for studying tissue structure, physiology, and function. Similar to other nonlinear optical imaging techniques, SRS is severely limited in imaging depth due to the turbidity and heterogeneity of tissue, regardless of whether imaging in the transmissive or epi mode. While this challenge is well known, important imaging parameters (namely maximum imaging depth and imaging signal to noise ratio) have rarely been reported in the literature. It is also important to compare epi mode and transmissive mode imaging to determine the best geometry for many tissue imaging applications. In this manuscript we report the achievable signal sizes and imaging depths using a simultaneous epi/transmissive imaging approach in four different murine tissues; brain, lung, kidney, and liver. For all four cases we report maximum signal sizes, scattering lengths, and achievable imaging depths as a function of tissue type and sample thickness. We report that for murine brain samples thinner than 2 mm transmissive imaging provides better results, while samples 2 mm and thicker are best imaged with epi imaging. We also demonstrate the use of a CNN-based denoising algorithm to yield a 40 µm (24%) increase in achievable imaging depth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.