ArticleFrontiers in genetics2020
NanoGBS: A Miniaturized Procedure for GBS Library Preparation.
Article in Frontiers in genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 31 citations in OpenAlex.
- SimGBS: a rapid method for simulating large-scale genotyping-by-sequencing data.BMC bioinformatics · 2025Article
- Dissecting genetic architecture of flowering and maturity traits in soybean using GWAS in Indian environment.BMC plant biology · 2025Article
- Unveiling key genetic determinants of charcoal rot resistance in soybean via genome-wide association studies.Frontiers in plant science · 2025Article
- Parentage assignment in black soldier fly (Frontiers in genetics · 2025Article
- Genetic insights into agronomic and morphological traits of drug-type cannabis revealed by genome-wide association studies.Scientific reports · 2024Article
- Comparing DNA isolation methods for forest trees: quality, plastic footprint, and time-efficiency.Plant methods · 2023Article
- 3D-GBS: a universal genotyping-by-sequencing approach for genomic selection and other high-throughput low-cost applications in species with small to medium-sized genomes.Plant methods · 2023Article
- Genotyping by Sequencing Advancements in Barley.Frontiers in plant science · 2022Review
- Genome-Wide Association Study of Soybean Germplasm Derived From Canadian × Chinese Crosses to Mine for Novel Alleles to Improve Seed Yield and Seed Quality Traits.Frontiers in plant science · 2022Article
- A bumper crop of SNPs in soybean through high-density genotyping-by-sequencing (HD-GBS).Plant biotechnology journal · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-throughput reduced-representation sequencing (RRS)-based genotyping methods, such as genotyping-by-sequencing (GBS), have provided attractive genotyping solutions in numerous species. Here, we present NanoGBS, a miniaturized and eco-friendly method for GBS library construction. Using acoustic droplet ejection (ADE) technology, NanoGBS libraries were constructed in tenfold smaller volumes compared to standard methods (StdGBS) and leading to a reduced use of plastics of up to 90%. A high-quality DNA library and SNP catalogue were obtained with extensive overlap (96%) in SNP loci and 100% agreement in genotype calls compared to the StdGBS dataset with a high level of accuracy (98.5%). A highly multiplexed pool of GBS libraries (768-plex) was sequenced on a single Ion Proton PI chip and yielded enough SNPs (~4K SNPs; 1.5 SNP per cM, on average) for many high-volume applications. Combining NanoGBS library preparation and increased multiplexing can dramatically reduce (72%) genotyping cost per sample. We believe that this approach will greatly facilitate the adoption of marker applications where extremely high throughputs are required and cost is still currently limiting.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.