ArticleFrontiers in immunology2020
Imaging Flow Cytometry Protocols for Examining Phagocytosis of Microplastics and Bioparticles by Immune Cells of Aquatic Animals.
Article in Frontiers in immunology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- Fluorescent Labeling Strategies for Tracking Micro- and Nanoplastics in Biological Systems.Materials (Basel, Switzerland) · 2026Review
- Immune response in honey bees (Apis mellifera) following humic substance feeding.Veterinary research communications · 2026Article
- Setup of a Simple and Cost-Effective pH-Sensitive Assay to Evaluate Phagocytosis in Rainbow Trout (Animals : an open access journal from MDPI · 2026Article
- β-Glucan training induces stimulus-dependent immune and metabolic modulation in head kidney leukocytes that persists for several days in Atlantic salmon (Frontiers in immunology · 2026Article
- Subcellular glycan-mannose receptor binding kinetics correlate with myeloid cell function.Nature communications · 2025Article
- Immunogenicity and protective efficacy of a native omp34 subunit vaccine againstVeterinary world · 2025Article
- Article
- Peptide-Conjugated Vascular Endothelial Extracellular Vesicles Encapsulating Vinorelbine for Lung Cancer Targeted Therapeutics.Nanomaterials (Basel, Switzerland) · 2024Article
- Distinct targeting and uptake of platelet and red blood cell-derived extracellular vesicles into immune cells.Journal of extracellular biology · 2024Article
- Quantitative Analysis of Latex Beads Phagocytosis by Human Macrophages Using Imaging Flow Cytometry with Extended Depth of Field (EDF).Methods in molecular biology (Clifton, N.J.) · 2023Article
- Continued P2X7 activation leads to mitochondrial fission and compromising microglial phagocytosis after subarachnoid haemorrhage.Journal of neurochemistry · 2022Article
- Exposure to microplastics leads to a defective ovarian function and change in cytoskeleton protein expression in rat.Environmental science and pollution research international · 2022Article
- Dietary Supplementation with γ-Aminobutyric Acid Improves Growth, Digestive Enzyme Activity, Non-Specific Immunity and Disease Resistance againstAnimals : an open access journal from MDPI · 2022Article
- Assessing Fish Immunotoxicity by Means ofFrontiers in immunology · 2022Review
- Imaging Flow Cytometry to Study Biofilm-Associated Microbial Aggregates.Molecules (Basel, Switzerland) · 2021Article
- Optimisation of phagocytosis assay in rainbow trout (Veterinarni medicina · 2021Article
- Macrophage Heterogeneity in the Intestinal Cells of Salmon: Hints From Transcriptomic and Imaging Data.Frontiers in immunology · 2021Article
- Adherent Intestinal Cells From Atlantic Salmon Show Phagocytic Ability and Express Macrophage-Specific Genes.Frontiers in cell and developmental biology · 2020Article
- Nutrient Digestibility, Growth, Mucosal Barrier Status, and Activity of Leucocytes From Head Kidney of Atlantic Salmon Fed Marine- or Plant-Derived Protein and Lipid Sources.Frontiers in immunology · 2020Article
- Intestinal Transcriptome Analysis Reveals Soy Derivative-Linked Changes in Atlantic Salmon.Frontiers in immunology · 2020Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Imaging flow cytometry (IFC) is a powerful tool which combines flow cytometry with digital microscopy to generate quantitative high-throughput imaging data. Despite various advantages of IFC over standard flow cytometry, widespread adoption of this technology for studies in aquatic sciences is limited, probably due to the relatively high equipment cost, complexity of image analysis-based data interpretation and lack of core facilities with trained personnel. Here, we describe the application of IFC to examine phagocytosis of particles including microplastics by cells from aquatic animals. For this purpose, we studied (1) live/dead cell assays and identification of cell types, (2) phagocytosis of degradable and non-degradable particles by Atlantic salmon head kidney cells and (3) the effect of incubation temperature on phagocytosis of degradable particles in three aquatic animals-Atlantic salmon, Nile tilapia, and blue mussel. The usefulness of the developed method was assessed by evaluating the effect of incubation temperature on phagocytosis. Our studies demonstrate that IFC provides significant benefits over standard flow cytometry in phagocytosis measurement by allowing integration of morphometric parameters, especially while identifying cell populations and distinguishing between different types of fluorescent particles and detecting their localization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.