ArticleFrontiers in physiology2020
Determination of Chitin Content in Insects: An Alternate Method Based on Calcofluor Staining.
Article in Frontiers in physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 68 citations in OpenAlex.
- Complexity of leaf surface texture affects microbial colonization in temperate forest tree species.PloS one · 2026Article
- Chitin Assessment in Insect-Based Products from Reference Methods to Near-Infrared Models.Insects · 2025Article
- Process optimisation for improved chitinase production from marine isolate Bacillus haynesii and bioethanol production with Saccharomyces cerevisiae.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Green Processes for Chitin and Chitosan Production from Insects: Current State, Challenges, and Opportunities.Polymers · 2025Review
- Analysis, Properties, and Applications of Insect-Derived Chitosan: A Sustainable Path to Functional Polysaccharide Materials.Gels (Basel, Switzerland) · 2025Review
- Profilin Pfy1 is critical for cell wall integrity and virulence inMicrobiology spectrum · 2025Article
- Chitin Synthase Is Required for Cuticle Formation and Molting in the Chinese Mitten CrabInternational journal of molecular sciences · 2025Article
- The Coexistence ofInternational journal of molecular sciences · 2024Article
- Chitinase inhibition induces transcriptional dysregulation altering ecdysteroid-mediated control ofiScience · 2024Article
- From Food Waste to Functional Biopolymers: Characterization of Chitin and Chitosan Produced from Prepupae of Black Soldier Fly Reared with Different Food Waste-Based Diets.Foods (Basel, Switzerland) · 2024Article
- Fluorescein-derived carbon dots with chitin-targeting for ultrafast and superstable fluorescent imaging of fungi.Nanophotonics (Berlin, Germany) · 2022Article
- Cuticle Modifications and Over-Expression of the Chitin-Synthase Gene in Diflubenzuron-Resistant Phenotype.Insects · 2022Article
- Physicochemical Characteristics of Biochar from Waste Cricket Chitin (Molecules (Basel, Switzerland) · 2022Article
- Evidencing New Roles for the Glycosyl-Transferase Cps1 in the Phytopathogenic FungusJournal of fungi (Basel, Switzerland) · 2022Article
- Insects in Pet Food Industry-Hope or Threat?Animals : an open access journal from MDPI · 2022Review
- Probing the antioxidant activity of functional proteins and bioactive peptides in Hermetia illucens larvae fed with food wastes.Scientific reports · 2022Article
- Nutritional composition, heavy metal content andFrontiers in nutrition · 2022Article
- Fluorescent Microscopy-Based Detection of Chitin in IntactFrontiers in physiology · 2022Article
- Essential Roles of Two FRQ Proteins (Frq1 and Frq2) in Beauveria bassiana's Virulence, Infection Cycle, and Calcofluor-Specific Signaling.Applied and environmental microbiology · 2021Article
- Application status and technical analysis of chitosan-based medical dressings: a review.RSC advances · 2020Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chitin is an aminopolysaccharide present in yeast cells and arthropod cuticle and is one of the most abundant biopolymers. The conventional methods for the quantitation of chitin content in biological samples are based on its hydrolysis (acid or enzymatic), and the assessment of the byproduct, glucosamine. However, previously described methodologies are time-consuming, laborious, low throughput, and not applicable to insect samples in many cases. Here we describe a new approach to chitin content quantitation based on calcofluor fluorescent brightener staining of samples, followed by microplate fluorescence readings. Calcofluor is a specific chitin stain commonly used for topological localization of the polymer. The protocol was tested in three important disease vector species, namely
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.