Evidence map›Paper›PMID 32132530›Full record

ArticleNature communications2020

Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9.

Oliver Xiaoou Dong, Shu Yu, Rashmi Jain, Nan Zhang, Phat Q Duong, Corinne Butler, Yan Li, Anna Lipzen, Joel A Martin, Kerrie W Barry and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers.

0numbers the graph read from it
0cells of the map it votes in
115citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

115 citing papers in PubMed.

  1. Review
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  4. Article
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  6. Review
  7. Article
  8. Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026
    Review
  9. Review
  10. Article
  11. Article
  12. Review
  13. Review
  14. Review
  15. Review
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article

55 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Oliver Xiaoou DongDepartment of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.ORCID http://orcid.org/0000-0003-4692-8659
Shu YuDepartment of Plant Sciences, University of California, Davis, CA, 95616, USA.
Rashmi JainDepartment of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.ORCID http://orcid.org/0000-0001-6819-847X
Nan ZhangDepartment of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.
Phat Q DuongDepartment of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.
Corinne ButlerDepartment of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.
Yan LiDepartment of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA.
Anna LipzenDepartment of Energy Joint Genome Institute, Berkeley, CA, 94720, USA.
Joel A MartinDepartment of Energy Joint Genome Institute, Berkeley, CA, 94720, USA.ORCID http://orcid.org/0000-0001-9511-6441
Kerrie W BarryDepartment of Energy Joint Genome Institute, Berkeley, CA, 94720, USA.
Jeremy SchmutzDepartment of Energy Joint Genome Institute, Berkeley, CA, 94720, USA.ORCID http://orcid.org/0000-0001-8062-9172
Li TianDepartment of Plant Sciences, University of California, Davis, CA, 95616, USA.ORCID http://orcid.org/0000-0001-6461-6072
Pamela C RonaldDepartment of Plant Pathology and the Genome Center, University of California, Davis, CA, 95616, USA. pcronald@ucdavis.edu.ORCID http://orcid.org/0000-0002-4107-1345

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Targeted insertion of transgenes at pre-determined plant genomic safe harbors provides a desirable alternative to insertions at random sites achieved through conventional methods. Most existing cases of targeted gene insertion in plants have either relied on the presence of a selectable marker gene in the insertion cassette or occurred at low frequency with relatively small DNA fragments (<1.8 kb). Here, we report the use of an optimized CRISPR-Cas9-based method to achieve the targeted insertion of a 5.2 kb carotenoid biosynthesis cassette at two genomic safe harbors in rice. We obtain marker-free rice plants with high carotenoid content in the seeds and no detectable penalty in morphology or yield. Whole-genome sequencing reveals the absence of off-target mutations by Cas9 in the engineered plants. These results demonstrate targeted gene insertion of marker-free DNA in rice using CRISPR-Cas9 genome editing, and offer a promising strategy for genetic improvement of rice and other crops.

Indexed as

Biosynthetic PathwaysCarotenoidsCRISPR-Cas SystemsDNA, PlantGene EditingGene Knock-In TechniquesGenome, PlantOryzaPlant BreedingPlants, Genetically ModifiedSeedsWhole Genome SequencingCarotenoidsDNA, Plant

Identifiers

PMID32132530
PMCPMC7055238

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.