ArticleMalaria journal2020
Assessment of subpatent Plasmodium infection in northwestern Ethiopia.
Article in Malaria journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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20 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Systematic review of sporozoite infection rate of Anopheles mosquitoes in Ethiopia, 2001-2021.Parasites & vectors · 2023Pooled it
- Trial
- Diagnostic challenges in malaria detection: A comparative diagnostic performance of HRP2-based rapid diagnostic tests, microscopy, and PCR at Bichena primary hospital, Northwest Ethiopia.Parasite epidemiology and control · 2026Article
- Asymptomatic/submicroscopicParasite epidemiology and control · 2025Article
- Utility of rapid diagnostic tests and microscopy to detect malaria in health facilities across the Solomon Islands.Malaria journal · 2025Article
- The burden and diagnostic challenges of subclinical Plasmodium falciparum infections in Southern Ghana.BMC infectious diseases · 2025Article
- Prevalence of subpatent Plasmodium falciparum infections in regions with varying transmission intensities and implications for malaria elimination in Mainland Tanzania.Malaria journal · 2025Article
- Article
- The potential of addressing asymptomatic malaria in the context of malaria elimination in Ethiopia: Scoping review.Public health in practice (Oxford, England) · 2023Article
- Asymptomatic and submicroscopic malaria infections in sugar cane and rice development areas of Ethiopia.Malaria journal · 2023Article
- Impact of Sub-patent Malaria During Pregnancy on Birth-Weight in Odisha, India: Time-to-Event Analysis of Prospective Longitudinal Follow-Up of a Survey.Journal of epidemiology and global health · 2023Article
- Performance Evaluation of Nested Polymerase Chain Reaction (Nested PCR), Light Microscopy, andPathogens (Basel, Switzerland) · 2022Article
- Health care provider practices in diagnosis and treatment of malaria in rural communities in Kisumu County, Kenya.Malaria journal · 2022Article
- Spatial Distribution of Plasmodium falciparum and Plasmodium vivax in Northern Ethiopia by Microscopic, Rapid Diagnostic Test, Laboratory Antibody, and Antigen Data.The Journal of infectious diseases · 2022Article
- qPCR in a suitcase for rapid Plasmodium falciparum and Plasmodium vivax surveillance in Ethiopia.PLOS global public health · 2022Article
- Rationalization of the Laboratory Diagnosis for Good Management of Malaria: Lessons from Transitional Methods.Journal of tropical medicine · 2022Article
- Article
- Purification of native histidine-rich protein 2 (nHRP2) from Plasmodium falciparum culture supernatant, infected RBCs, and parasite lysate.Malaria journal · 2021Article
- The epidemiology and detectability of asymptomatic plasmodium vivax and plasmodium falciparum infections in low, moderate and high transmission settings in Ethiopia.Malaria journal · 2021Article
- Antibodies Against theFrontiers in cellular and infection microbiology · 2021Article
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Authors and funding
19 authors at 10 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEthiopia has set a goal for malaria elimination by 2030. Low parasite density infections may go undetected by conventional diagnostic methods (microscopy and rapid diagnostic tests) and their contribution to malaria transmission varies by transmission settings. This study quantified the burden of subpatent infections from samples collected from three regions of northwest Ethiopia.
methodsSub-samples of dried blood spots from the Ethiopian Malaria Indicator Survey 2015 (EMIS-2015) were tested and compared using microscopy, rapid diagnostic tests (RDTs), and nested polymerase chain reaction (nPCR) to determine the prevalence of subpatent infection. Paired seroprevalence results previously reported along with gender, age, and elevation of residence were explored as risk factors for Plasmodium infection.
resultsOf the 2608 samples collected, the highest positive rate for Plasmodium infection was found with nPCR 3.3% (95% CI 2.7-4.1) compared with RDT 2.8% (95% CI 2.2-3.5) and microscopy 1.2% (95% CI 0.8-1.7). Of the nPCR positive cases, Plasmodium falciparum accounted for 3.1% (95% CI 2.5-3.8), Plasmodium vivax 0.4% (95% CI 0.2-0.7), mixed P. falciparum and P. vivax 0.1% (95% CI 0.0-0.4), and mixed P. falciparum and Plasmodium malariae 0.1% (95% CI 0.0-0.3). nPCR detected an additional 30 samples that had not been detected by conventional methods. The majority of the nPCR positive cases (61% (53/87)) were from the Benishangul-Gumuz Region. Malaria seropositivity had significant association with nPCR positivity [adjusted OR 10.0 (95% CI 3.2-29.4), P < 0.001].
conclusionUsing nPCR the detection rate of malaria parasites increased by nearly threefold over rates based on microscopy in samples collected during a national cross-sectional survey in 2015 in Ethiopia. Such subpatent infections might contribute to malaria transmission. In addition to strengthening routine surveillance systems, malaria programmes may need to consider low-density, subpatent infections in order to accelerate malaria elimination efforts.
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