ArticleBMC genomics2020
Scale development and utilization of universal PCR-based and high-throughput KASP markers specific for chromosome arms of rye (Secale cereale L.).
Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 33 citations in OpenAlex.
- Identification of KASP markers and putative genes for herbicide (metribuzin) tolerance in common wheat (Triticum aestivum L.).BMC plant biology · 2026Article
- Genetic dissection and validation of major and stable QTLs for the flag leaf size-related traits in common wheat (Triticum aestivum L.).BMC plant biology · 2026Article
- Cytogenetic characterization of a novel wheat-rye 2R (2D) substitution line YT9 conferring powdery mildew resistance from the three-leaf stage and physical mapping of PmYT9.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Establishment of a set of St-group wheat-Frontiers in plant science · 2025Article
- Development and Characterization of Two Wheat-Rye Introgression Lines with Resistance to Stripe Rust and Powdery Mildew.International journal of molecular sciences · 2024Article
- Genome-wide simple sequence repeat analysis and specific molecular marker development of rye.BMC genomics · 2024Article
- Two functional CC-NBS-LRR proteins from rye chromosome 6RS confer differential age-related powdery mildew resistance to wheat.Plant biotechnology journal · 2024Article
- Fighting wheat powdery mildew: from genes to fields.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Identification of KASP markers and candidate genes for drought tolerance in wheat using 90K SNP array genotyping of near-isogenic lines targeting a 4BS quantitative trait locus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Development of novel wheat-rye 6RS small fragment translocation lines with powdery mildew resistance and physical mapping of the resistance gene PmW6RS.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Effectiveness ofInternational journal of molecular sciences · 2023Article
- Construction of a high-density genetic map and mapping of a spike length locus for rye.PloS one · 2023Article
- Chromosome-specific KASP markers for detecting Amblyopyrum muticum segments in wheat introgression lines.The plant genome · 2022Article
- Molecular Cytogenetic Identification of a New Wheat-Rye 6R Addition Line and Physical Localization of Its Powdery Mildew Resistance Gene.Frontiers in plant science · 2022Article
- Cytogenetic identification and molecular marker development for the novel stripe rust-resistant wheat-aBIOTECH · 2021Article
- Transcriptome analysis and genetic diversity ofMitochondrial DNA. Part B, Resources · 2021Article
- New Transcriptome-Based SNP Markers for Noug (Genes · 2020Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundRye (Secale cereale L., 2n = 2x = 14, RR), a relative of common wheat, is a large gene resource pool for wheat improvement. Accurate and convenient identification of the rye chromatin in wheat background will facilitate the transfer and utilization of elite genes derived from rye in wheat breeding.
resultsIn the present study, five rye cultivars including Imperial, German White, Jingzhouheimai, Baili and Guyuan were sequenced by specific-locus amplified fragment sequencing (SLAF-seq) to develop large-scale rye-specific markers. Based on SLAF-seq and bioinformatics analyses, a total of 404 universal PCR-based and a whole set of Kompetitive allele-specific PCR (KASP) markers specific for the 14 individual rye chromosome arms were developed and validated. Additionally, two KASP markers specific for 1RS and 2RL were successfully applied in the detection of 1RS translocations in a natural population and 2RL chromosome arms in wheat-rye derived progenies that conferred adult resistance to powdery mildew.
conclusionThe 404 PCR-based markers and 14 KASP markers specific for the 14 individual rye chromosome arms developed in this study can enrich the marker densities for gene mapping and accelerate the utilization of rye-derived genes in wheat improvement. Especially, the KASP markers achieved high-throughput and accurate detection of rye chromatin in wheat background, thus can be efficiently used in marker-assisted selection (MAS). Besides, the strategy of rye-specific PCR-based markers converting into KASP markers was high-efficient and low-cost, which will facilitate the tracing of alien genes, and can also be referred for other wheat relatives.
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