Evidence map›Paper›PMID 32130536›Full record

ArticleMikrochimica acta2020

A molecule capturer analysis system for visual determination of avian pathogenic Escherichia coli serotype O78 using a lateral flow assay.

Wenfang Nie, Juanfang Wang, Jianguo Xu, Li Yao, Dongqing Qiao, Feng Xue, Fang Tang, Wei Chen

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Virulence Determinants and Multidrug Resistance ofAntibiotics (Basel, Switzerland) · 2021
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Wenfang NieMOE Joint International Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
Juanfang WangMOE Joint International Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
Jianguo XuSchool of Food Science & Biological Engineering, Engineering Research Center of Bio-process, MOE, Hefei University of Technology, Hefei, 230009, China.
Li YaoSchool of Food Science & Biological Engineering, Engineering Research Center of Bio-process, MOE, Hefei University of Technology, Hefei, 230009, China.
Dongqing QiaoSchool of Food Science & Biological Engineering, Engineering Research Center of Bio-process, MOE, Hefei University of Technology, Hefei, 230009, China.
Feng XueMOE Joint International Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
Fang TangMOE Joint International Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China. tfalice@126.com.
Wei ChenSchool of Food Science & Biological Engineering, Engineering Research Center of Bio-process, MOE, Hefei University of Technology, Hefei, 230009, China. chenweishnu@163.com.ORCID http://orcid.org/0000-0003-3763-1183
Hefei University of Technology · CNNanjing Agricultural University · CN

Funding

China Agricultural Research System CARS-48MOST 2016YDF0500800 & 2017YFF0208600
6 · The paper itself

Abstract

A method for rapid and accurate determination of avian pathogenic Escherichia coli serotype O78 (APEC O78) by the gold nanoparticle-labeled lateral flow strip method, entitled molecule capturer analysis system (MCAS), is described. Target virulence-associated gene of APEC O78 is adopted as the analyte. After pre-amplification with the designed functional primer set, numerous new-formed amplicons are simultaneously labeled with fluorescein isothiocyanate (FITC) and digoxin. AuNPs with a diameter of 18 nm and the characteristic plasmonic peak at 526 nm are utilized for labeling. These two labels of FITC and digoxin are further captured and measured with the AuNP-labeled lateral flow strip, and the AuNPs are retained on the test line through the immunoreaction for signal output. Under optimized conditions, this MCAS protocol can determine the target APEC O78 with excellent determination limit of 4.3 cfu mL

Indexed as

Bacteriological TechniquesAnimalsChickensEscherichia coliAvian pathogenic Escherichia coliDigoxinDual-labelingGold nanoparticleLateral flow stripMolecular amplificationMolecule capturingRapid assay

Identifiers

PMID32130536
OpenAlexW3009880722

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.