ArticlePloS one2020
A survey of extended-spectrum beta-lactamase-producing Enterobacteriaceae in urban wetlands in southwestern Nigeria as a step towards generating prevalence maps of antimicrobial resistance.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- Antibiotic resistance genes circulating in Nigeria: a systematic review and meta-analysis from the One Health perspective.BMC medical genomics · 2025Pooled it
- Genomic profiling of extended-spectrum beta-lactamase-producing Escherichia coli isolated from poultry and poultry farm workers in Accra, Ghana.Scientific reports · 2026Article
- Environmental Dissemination of Multidrug-Resistant Gram-Negative Pathogens in Djibouti's Wastewaters.Public health challenges · 2025Article
- Environmental Risk Factors Contributing to the Spread of Antibiotic Resistance in West Africa.Microorganisms · 2025Review
- Sustainable agriculture: leveraging microorganisms for a circular economy.Applied microbiology and biotechnology · 2024Review
- Solid waste dumpsite leachate and contiguous surface water contain multidrug-resistant ESBL-producing Escherichia coli carrying Extended Spectrum β-Lactamase (ESBL) genes.BMC microbiology · 2024Article
- Molecular characterization and prevalence of β-lactamase-producing Enterobacterales in livestock and poultry slaughterhouses wastewater in Iran.Journal of water and health · 2024Article
- Large-Scale Biogeographical Shifts of Abundance of Antibiotic Resistance Genes and Marine Bacterial Communities as Their Carriers along a Trophic Gradient.International journal of molecular sciences · 2024Article
- Article
- Molecular Characterization of Antibiotic Resistance Determinants inAntibiotics (Basel, Switzerland) · 2023Article
- The fate of sulfonamide resistance genes and anthropogenic pollution markerFrontiers in microbiology · 2023Article
- A cross-sectional questionnaire survey on knowledge of anti-protozoal drug use and resistance among AHPs in Kwara State, Nigeria.BMC veterinary research · 2022Article
- Genomic Insights of Multidrug-ResistantFrontiers in veterinary science · 2021Article
- Raw Cow Milk Bacterial Consortium as Bioindicator of Circulating Anti-Microbial Resistance (AMR).Animals : an open access journal from MDPI · 2020Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In many countries, emission of insufficiently treated wastewater into water bodies appears to be an important factor in spreading clinically relevant antimicrobial resistant bacteria. In this study, we looked for the presence of Enterobacteriaceae strains with resistance to 3rd generation cephalosporin antibiotics in four urban wetlands in southwestern Nigeria by isolation, whole genome sequencing and qPCR enumeration of marker genes. Genome analysis of multi-drug resistant and potentially pathogenic Escherichia coli isolates (members of the widely distributed ST10 complex) revealed the presence of the extended spectrum beta-lactamase gene blaCTX-M-15 on self-transmissible IncF plasmids. The gene was also present together with a blaTEM-1B gene on self-transmissible IncH plasmids in multi-drug resistant Enterobacter cloacae isolates. A Citrobacter freundii isolate carried blaTEM-1B on an IncR-type plasmid without discernable conjugation apparatus. All strains were isolated from a wetland for which previous qPCR enumeration of marker genes, in particular the ratio of intI1 to 16S rRNA gene copy numbers, had indicated a strong anthropogenic impact. Consistent with the isolation origin, qPCR analysis in this study showed that the blaCTX-M gene was present at an abundance of 1x10-4 relative to bacterial 16S rRNA gene copy numbers. The results indicate that contamination of these urban aquatic ecosystems with clinically relevant antibiotic resistant bacteria is substantial in some areas. Measures should therefore be put in place to mitigate the propagation of clinically relevant antimicrobial resistance within the Nigerian aquatic ecosystems.
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