ArticleBiotechnology for biofuels2020
Improvement of the catalytic activity and thermostability of a hyperthermostable endoglucanase by optimizing N-glycosylation sites.
Article in Biotechnology for biofuels, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Regulating protein glycosylation modification enhances the synthesis of taxadiene inSynthetic and systems biotechnology · 2026Article
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- Efficient biodegradation and upcycling of polyethylene terephthalate mediated by cell-factories.Frontiers in microbiology · 2025Review
- Improved Thermal Stability of a Novel Acidophilic Phytase.Journal of microbiology and biotechnology · 2024Article
- Enhanced crystalline cellulose degradation by a novel metagenome-derived cellulase enzyme.Scientific reports · 2024Article
- An impact of N-glycosylation on biochemical properties of a recombinant α-amylase fromHeliyon · 2024Article
- Structural basis of EHEP-mediated offense against phlorotannin-induced defense from brown algae to protecteLife · 2023Article
- Analyzing Current Trends and Possible Strategies to Improve Sucrose Isomerases' Thermostability.International journal of molecular sciences · 2023Review
- Glycosylation Contributes to Thermostability and Proteolytic Resistance of rFIP-nha (Molecules (Basel, Switzerland) · 2023Article
- Functional tailoring of a PET hydrolytic enzyme expressed in Pichia pastoris.Bioresources and bioprocessing · 2023Article
- Multicopper oxidases with laccase-ferroxidase activity: Classification and study of ferroxidase activity determinants in a member fromComputational and structural biotechnology journal · 2023Article
- Expression, characterization, and activity optimization of a novel cellulase from the thermophilic bacteria Cohnella sp. A01.Scientific reports · 2022Article
- Enhancing the Thermostability of Phytase to Boiling Point by Evolution-Guided Design.Applied and environmental microbiology · 2022Article
- Fungal cellulases: protein engineering and post-translational modifications.Applied microbiology and biotechnology · 2022Review
- Improved methanol tolerance of Rhizomucor miehei lipase based on N‑glycosylation within the α-helix region and its application in biodiesel production.Biotechnology for biofuels · 2021Article
- Heterologous Expression, Engineering and Characterization of a Novel Laccase ofJournal of fungi (Basel, Switzerland) · 2021Article
- Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris.Biotechnology for biofuels · 2021Article
- Design and High Expression of Non-glycosylated Lysostaphins inFrontiers in microbiology · 2021Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEndoglucanase has been extensively employed in industrial processes as a key biocatalyst for lignocellulosic biomass degradation. Thermostable endoglucanases with high catalytic activity at elevated temperatures are preferred in industrial use. To improve the activity and thermostability, site-directed mutagenesis was conducted to modify the N-glycosylation sites of the thermostable β-1,4-endoglucanase CTendo45 from
resultsIn this study, structure-based rational design was performed based on the modification of N-glycosylation sites in CTendo45. Eight single mutants and one double mutant were constructed and successfully expressed in
conclusionsThis study provides initial insight into the biochemical function of N-glycosylation in thermostable endoglucanases. Moreover, the design approach to the optimization of N-glycosylation sites presents an effective and feasible strategy to improve enzymatic activity and thermostability.
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