ArticlePLoS biology2020
A G protein-coupled receptor mediates neuropeptide-induced oocyte maturation in the jellyfish Clytia.
Article in PLoS biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 51 citations in OpenAlex.
- Revisiting nerve nets: functional organization and evolutionary implications.The EMBO journal · 2026Review
- A light-entrained clock mechanism in a hydrozoan jellyfish synchronizes evening gamete release.PLoS biology · 2026Article
- Gender-specific function of melatonin receptor (MTRI) in gonads of red swamp crayfish, Procambarus clarkii.Scientific reports · 2025Article
- Luteinizing hormone-induced changes in the structure of mammalian preovulatory follicles.Current topics in developmental biology · 2025Review
- Dynamics of neural activity in early nervous system evolution.Current opinion in behavioral sciences · 2024Article
- Jellyfish for the study of nervous system evolution and function.Current opinion in neurobiology · 2024Review
- Large-scale deorphanization ofeLife · 2024Article
- Storage cell proliferation during somatic growth establishes that tardigrades are not eutelic organisms.Biology open · 2024Article
- Identification and Characterization ofBiomolecules · 2023Article
- Conserved meiotic mechanisms in the cnidarianScience advances · 2023Article
- siRNA-mediated gene knockdown via electroporation in hydrozoan jellyfish embryos.Scientific reports · 2022Article
- Molecular and cellular architecture of the larval sensory organ in the cnidarian Nematostella vectensis.Development (Cambridge, England) · 2022Article
- Premetazoan Origin of Neuropeptide Signaling.Molecular biology and evolution · 2022Article
- Review: The evolution of peptidergic signaling in Cnidaria and Placozoa, including a comparison with Bilateria.Frontiers in endocrinology · 2022Review
- An evolutionary genomics view on neuropeptide genes in Hydrozoa and Endocnidozoa (Myxozoa).BMC genomics · 2021Article
- Nemertean, Brachiopod, and Phoronid Neuropeptidomics Reveals Ancestral Spiralian Signaling Systems.Molecular biology and evolution · 2021Article
- Selective dephosphorylation by PP2A-B55 directs the meiosis I-meiosis II transition in oocytes.eLife · 2021Article
- Cellular pathways during spawning induction in the starlet sea anemone Nematostella vectensis.Scientific reports · 2021Article
- The chemical brain hypothesis for the origin of nervous systems.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Article
- Identifying Receptors for Neuropeptides and Peptide Hormones: Challenges and Recent Progress.ACS chemical biology · 2021Review
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The reproductive hormones that trigger oocyte meiotic maturation and release from the ovary vary greatly between animal species. Identification of receptors for these maturation-inducing hormones (MIHs) and understanding how they initiate the largely conserved maturation process remain important challenges. In hydrozoan cnidarians including the jellyfish Clytia hemisphaerica, MIH comprises neuropeptides released from somatic cells of the gonad. We identified the receptor (MIHR) for these MIH neuropeptides in Clytia using cell culture-based "deorphanization" of candidate oocyte-expressed G protein-coupled receptors (GPCRs). MIHR mutant jellyfish generated using CRISPR-Cas9 editing had severe defects in gamete development or in spawning both in males and females. Female gonads, or oocytes isolated from MIHR mutants, failed to respond to synthetic MIH. Treatment with the cAMP analogue Br-cAMP to mimic cAMP rise at maturation onset rescued meiotic maturation and spawning. Injection of inhibitory antibodies to the alpha subunit of the Gs heterodimeric protein (GαS) into wild-type oocytes phenocopied the MIHR mutants. These results provide the molecular links between MIH stimulation and meiotic maturation initiation in hydrozoan oocytes. Molecular phylogeny grouped Clytia MIHR with a subset of bilaterian neuropeptide receptors, including neuropeptide Y, gonadotropin inhibitory hormone (GnIH), pyroglutamylated RFamide, and luqin, all upstream regulators of sexual reproduction. This identification and functional characterization of a cnidarian peptide GPCR advances our understanding of oocyte maturation initiation and sheds light on the evolution of neuropeptide-hormone systems.
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