ArticlePLoS neglected tropical diseases2020
Distinct gene expression patterns in vector-residing Leishmania infantum identify parasite stage-enriched markers.
Article in PLoS neglected tropical diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Discovery of an Adaptive Neuroimmune Response Driving Itch and Fast Tick Removal with Implications for Preventing Pathogen Transmission.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Discovery of an Adaptive Neuroimmune Response Driving Itch and Fast Tick Removal with Implications for Preventing Pathogen Transmission.bioRxiv : the preprint server for biology · 2025Article
- The impact of blood on vector-borne diseases with emphasis on mosquitoes and sand flies.Trends in parasitology · 2025Review
- Single-cell atlas ofProceedings of the National Academy of Sciences of the United States of America · 2024Article
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- Biological effects ofFrontiers in cellular and infection microbiology · 2023Article
- Sand flies: Basic information on the vectors of leishmaniasis and their interactions with Leishmania parasites.Communications biology · 2022Review
- The effect of the sugar metabolism on Leishmania infantum promastigotes inside the gut of Lutzomyia longipalpis: A sweet relationship?PLoS neglected tropical diseases · 2022Article
- Finding Correlations Between mRNA and Protein Levels inFrontiers in cellular and infection microbiology · 2022Review
- Proteome and morphological analysis show unexpected differences between promastigotes of Leishmania amazonensis PH8 and LV79 strains.PloS one · 2022Article
- Paving the Way: Contributions of Big Data to Apicomplexan and Kinetoplastid Research.Frontiers in cellular and infection microbiology · 2022Review
- Replacement ofFrontiers in cellular and infection microbiology · 2022Article
- Application of single-cell transcriptomics to kinetoplastid research.Parasitology · 2021Review
- TheFrontiers in cellular and infection microbiology · 2021Article
- The gene expression of Leishmania infantum chagasi inside Lutzomyia longipalpis, the main vector of visceral leishmaniasis in Brazil.Memorias do Instituto Oswaldo Cruz · 2021Article
- Advances in UnderstandingFrontiers in cell and developmental biology · 2021Review
- Leishmania infection induces a limited differential gene expression in the sand fly midgut.BMC genomics · 2020Article
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6 authors.
Funding
Abstract
backgroundLeishmaniasis is a vector-borne neglected disease. Inside the natural sand fly vector, the promastigote forms of Leishmania undergo a series of extracellular developmental stages to reach the infectious stage, the metacyclic promastigote. There is limited information regarding the expression profile of L. infantum developmental stages inside the sand fly vector, and molecular markers that can distinguish the different parasite stages are lacking. METHODOLOGY/PRINCIPAL
findingsWe performed RNAseq on unaltered midguts of the sand fly Lutzomyia longipalpis after infection with L. infantum parasites. RNAseq was carried out at various time points throughout parasite development. Principal component analysis separated the transcripts corresponding to the different Leishmania promastigote stages, the procyclic, nectomonad, leptomonad and metacyclics. Importantly, there were a significant number of differentially expressed genes when comparing the sequential development of the various Leishmania stages in the sand fly. There were 836 differentially expressed (DE) genes between procyclic and long nectomonad promastigotes; 113 DE genes between nectomonad and leptomonad promastigotes; and 302 DE genes between leptomonad and metacyclic promastigotes. Most of the DE genes do not overlap across stages, highlighting the uniqueness of each Leishmania stage. Furthermore, the different stages of Leishmania parasites exhibited specific transcriptional enrichment across chromosomes. Using the transcriptional signatures exhibited by distinct Leishmania stages during their development in the sand fly midgut, we determined the genes predominantly enriched in each stage, identifying multiple potential stage-specific markers for L. infantum.
conclusionsOverall, these findings demonstrate the transcriptional plasticity of the Leishmania parasite inside the sand fly vector and provide a repertoire of potential stage-specific markers for further development as molecular tools for epidemiological studies.
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