ArticleGraefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie2020
Genipin inhibits the scleral expression of miR-29 and MMP2 and promotes COL1A1 expression in myopic eyes of guinea pigs.
Article in Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Correlation between axial length and the elasticity of the posterior segment of eyeball in myopic eyes without posterior staphyloma: a shear wave elastography-based cross-sectional study.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2025Article
- Research Progress of Epigenetic Modifications in Myopia.International journal of medical sciences · 2025Review
- The potential of brimonidine for myopia treatment: Targeting MMP-2 to regulate choroidal thickness and control eye growth.Heliyon · 2024Article
- Review
- Analysis and validation of potential ICD-related biomarkers in development of myopia using machine learning.International ophthalmology · 2024Article
- The Role of miR-29 Family in TGF-β Driven Fibrosis in Glaucomatous Optic Neuropathy.International journal of molecular sciences · 2022Review
- Scleral crosslinking using genipin can compromise retinal structure and function in tree shrews.Experimental eye research · 2022Article
- Novel Findings regarding the Bioactivity of the Natural Blue Pigment Genipin in Human Diseases.International journal of molecular sciences · 2022Review
- Non-coding RNAs and related molecules associated with form-deprivation myopia in mice.Journal of cellular and molecular medicine · 2022Article
- Intravitreal brimonidine inhibits form-deprivation myopia in guinea pigs.Eye and vision (London, England) · 2021Article
- Effect of Scleral Crosslinking Using Multiple Doses of Genipin on Experimental Progressive Myopia in Tree Shrews.Translational vision science & technology · 2021Article
- Transpupillary collagen photocrosslinking for targeted modulation of ocular biomechanics.Biomaterials · 2021Article
- Exosomal miR-223 derived from natural killer cells inhibits hepatic stellate cell activation by suppressing autophagy.Molecular medicine (Cambridge, Mass.) · 2020Article
- Exosomes derived from natural killer cells inhibit hepatic stellate cell activation and liver fibrosis.Human cell · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeHigh myopia can lead to blindness. Genipin is a collagen cross-linking agent that may be used to treat myopia. However, the mechanism of action of genipin for the treatment of myopia is unclear. This study investigated the effect of genipin on the scleral expression of the miR-29 cluster, matrix metalloproteinase 2 (MMP2), and collagen alpha1 chain of type I (COL1A1) in a guinea pig model of myopia.
methodsThe model of myopia was established by treating guinea pigs with a - 8D lens on both eyes for 21 days, and eyes with a refractive error of - 6D or greater were included. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) and western blot were used to examine the mRNA and protein expression, respectively. A dual-luciferase assay was used to determine the direct targeting of the miR-29 cluster on the 3'-untranslated region (UTR) of the COL1A1 gene.
resultsThe scleral expression of miR-29a, miR-29b, and miR-29c as well as MMP2 was significantly increased, and the scleral expression of COL1A1 was significantly decreased in the myopia group. Genipin treatment reversed these effects in myopic eyes. The dual-luciferase assay showed that the luciferase activities were significantly decreased in human embryonic kidney (HEK) cells transfected with miR-29a and miR-29b, but not miR-29c, compared with those transfected with control miRNAs.
conclusionsGenipin inhibits the scleral expression of the miR-29 cluster and MMP2 and promotes COL1A1 expression in a guinea pig model of myopia. Thus, genipin may promote COL1A1 expression by reducing the expression of the miR-29 cluster.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.