Evidence map›Paper›PMID 32125274›Full record

ArticleeLife2020

New approach for membrane protein reconstitution into peptidiscs and basis for their adaptability to different proteins.

Gabriella Angiulli, Harveer Singh Dhupar, Hiroshi Suzuki, Irvinder Singh Wason, Franck Duong Van Hoa, Thomas Walz

Abstract read
In one paragraph

Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.

0numbers the graph read from it
0cells of the map it votes in
43citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

43 citing papers in PubMed.

  1. Article
  2. Review
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  4. Article
  5. Article
  6. Review
  7. Review
  8. Article
  9. Article
  10. Review
  11. Review
  12. Nanodiscs for the study of membrane proteins.Current opinion in structural biology · 2024
    Review
  13. A Second Drug Binding Site in P2X3.bioRxiv : the preprint server for biology · 2024
    Article
  14. Comprehensive Characterization of LAT1 Cholesterol-Binding Sites.Journal of chemical theory and computation · 2024
    Article
  15. Article
  16. Article
  17. Article
  18. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Gabriella Angiulli *Laboratory of Molecular Electron Microscopy, Rockefeller University, New York, United States.ORCID 0000-0002-0092-9552
Harveer Singh Dhupar *Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada.
Hiroshi SuzukiLaboratory of Molecular Electron Microscopy, Rockefeller University, New York, United States.ORCID 0000-0001-5371-6385
Irvinder Singh WasonDepartment of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada.ORCID 0000-0002-3004-857X
Franck Duong Van HoaDepartment of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada.ORCID 0000-0001-7328-6124
Thomas WalzLaboratory of Molecular Electron Microscopy, Rockefeller University, New York, United States.ORCID 0000-0003-2606-2835

Funding

Natural Sciences and Engineering Research Council of Canada RGPIN-2016-04241
6 · The paper itself

Abstract

Previously we introduced peptidiscs as an alternative to detergents to stabilize membrane proteins in solution (Carlson et al., 2018). Here, we present 'on-gradient' reconstitution, a new gentle approach for the reconstitution of labile membrane-protein complexes, and used it to reconstitute

Indexed as

NanostructuresBacterial ProteinsCell MembraneCryoelectron MicroscopyGene Expression Regulation, BacterialImage Processing, Computer-AssistedMembrane ProteinsProtein ConformationRhodobacter sphaeroidesBacterial ProteinsMembrane Proteinsbiochemistrychemical biologyCryo-electron microscopyE. colimembrane mimeticsmolecular biophysicsnanodiscsPeptidiscsstructural biology

Identifiers

PMID32125274
PMCPMC7053995

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.