Evidence map›Paper›PMID 32125139›Full record

ArticleAnalytical chemistry2020

Miniaturized Filter-Aided Sample Preparation (MICRO-FASP) Method for High Throughput, Ultrasensitive Proteomics Sample Preparation Reveals Proteome Asymmetry in

Zhenbin Zhang, Kyle M Dubiak, Paul W Huber, Norman J Dovichi

Abstract read
In one paragraph

Article in Analytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Cell lineage-guided mass spectrometry reveals increased energy metabolism and reactive oxygen species in the vertebrate organizer.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  7. Fully Integrated Online Strategy for Highly Sensitive Proteome Profiling.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
  8. Article
  9. Article
  10. Article
  11. Bottom-Up Proteomics: Advancements in Sample Preparation.International journal of molecular sciences · 2023
    Review
  12. Separation methods in single-cell proteomics: RPLC or CE?International journal of mass spectrometry · 2022
    Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Zhenbin ZhangDepartment of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556 United States.
Kyle M DubiakDepartment of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556 United States.
Paul W HuberDepartment of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556 United States.
Norman J DovichiDepartment of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556 United States.ORCID 0000-0002-1331-3191

Funding

Advances in bioanalysisR35GM136334 · NIGMS · UNIVERSITY OF NOTRE DAME · PI DOVICHI, NORMAN J · 2020 to 2024
$2.0M
NIGMS NIH HHS R35 GM136334
6 · The paper itself

Abstract

We report a miniaturized filter aided sample preparation method (micro-FASP) for low-loss preparation of submicrogram proteomic samples. The method employs a filter with ∼0.1 mm

Indexed as

FiltrationLimit of DetectionProteomicsAnalytic Sample Preparation MethodsAnimalsCell CountEmbryo, NonmammalianMiniaturizationXenopus laevis

Identifiers

PMID32125139
PMCPMC7931810

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.