Evidence map›Paper›PMID 32124173›Full record

ArticleMolecular biology reports2020

Efficient isolation and proliferation of human adipose-derived mesenchymal stromal cells in xeno-free conditions.

Natalia Langenfeld Fuoco, Rafael Guilen de Oliveira, Monica Yonashiro Marcelino, Talita Stessuk, Marna Eliana Sakalem, Denis Aloisio Lopes Medina, Waldir Pereira Modotti, Andresa Forte, João Tadeu Ribeiro-Paes

Abstract read
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In one paragraph

Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Isolation and characterization of farm pig adipose tissue-derived mesenchymal stromal/stem cells.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2022
    Article
  6. Article
  7. Review
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Natalia Langenfeld FuocoBiotechnology Interunits Post-Graduation Program, Biomedical Science Institute, University of São Paulo (USP), São Paulo, SP, Brazil.
Rafael Guilen de OliveiraBiotechnology Interunits Post-Graduation Program, Biomedical Science Institute, University of São Paulo (USP), São Paulo, SP, Brazil.
Monica Yonashiro MarcelinoBiotechnology Interunits Post-Graduation Program, Biomedical Science Institute, University of São Paulo (USP), São Paulo, SP, Brazil.
Talita StessukBiotechnology Interunits Post-Graduation Program, Biomedical Science Institute, University of São Paulo (USP), São Paulo, SP, Brazil.
Marna Eliana SakalemGenetics and Cell Therapy Laboratory (GenTe Cel), São Paulo State University (Unesp), São Paulo, SP, Brazil.
Denis Aloisio Lopes MedinaInstitute of Medical-Hospital Service (IAM), São Paulo, SP, Brazil.
Waldir Pereira ModottiInstitute of Medical-Hospital Service (IAM), São Paulo, SP, Brazil.
Andresa ForteSão Lucas - Cell Therapy Group, São Paulo, SP, Brazil.
João Tadeu Ribeiro-PaesGenetics and Cell Therapy Laboratory (GenTe Cel), São Paulo State University (Unesp), São Paulo, SP, Brazil. ribeiro.paes@unesp.br.ORCID http://orcid.org/0000-0002-7645-5891

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Classical methods used for culture of adipose-derived mesenchymal stromal cells (ADSCs) use xenobiotic components, which may present a potential risk for biological contamination and/or elicit immunological reactions. Therefore, the aim of this study was to establish a xeno-free methodology for the isolation and proliferation of human ADSCs (hADSCs). hADSCs were isolated by enzymatic digestion or mechanical dissociation and cultured in the presence of fetal bovine serum or human platelet lysate. Proliferation curves were performed as a function of time from the cell culture and used to calculate the population doubling time. Immunophenotyping and differentiation tests were used to identify and characterize the hADSCs. Human ADSCs isolated and cultured in conventional or xenobiotic-free conditions peaked at different days but achieved similar maximum proliferation. The hADSCs differentiation ability was similar in all groups. The characterization of hADSCs by flow cytometry showed low contamination of the cultures by other cell types. The xenobiotic-free methodology described in this study is a feasible and reproducible alternative for isolation and proliferation of hADSCs. This methodology is in accordance with the recommendations of the National Health Surveillance Agency, which proposes avoidance of xenobiotic products.

Indexed as

Adipose TissueCell Culture TechniquesCell DifferentiationCell ProliferationCells, CulturedCulture MediaFlow CytometryHumansImmunophenotypingMesenchymal Stem CellsXenobioticsCulture MediaXenobioticsAdipose tissueFetal bovine serum (FBS)Mesenchymal stromal cellsPlatelet lysateStem cells

Identifiers

PMID32124173

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.