ArticleMolecular biology reports2020
Development of polymorphic EST-SSR markers and their applicability in genetic diversity evaluation in Rhododendron arboreum.
Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed.
- Investigating the genetic potential of Ashwagandha (Withania somnifera L. Dunal) germplasm through SCoT and EST-SSR molecular markers.Scientific reports · 2026Article
- Exploration of Genetic Variation and Population Structure in Bergenia ciliata for its Conservation Implications.Biochemical genetics · 2025Article
- Development of Single Nucleotide Polymorphism and Phylogenetic Analysis ofPlants (Basel, Switzerland) · 2025Article
- Review
- Contradistinctive floral attributes, pollination guilds and their consequence on the outcrossing rate in two elevational morphs ofFrontiers in plant science · 2024Article
- Genetic diversity, population structure, and DNA fingerprinting of Ailanthus altissima var. erythrocarpa based on EST-SSR markers.Scientific reports · 2023Article
- DBALM: A novel method for identifying ornamental flowering plants based on DNA barcodes-leaf morphology.Ecology and evolution · 2023Article
- A review on efforts for improvement in medicinally important chemical constituents in3 Biotech · 2023Review
- Improving Blueberry Fruit Nutritional Quality through Physiological and Genetic Interventions: A Review of Current Research and Future Directions.Antioxidants (Basel, Switzerland) · 2023Review
- Assessment of genetic diversity and population structure in wild Ziziphus species from northwest India using SSR marker technique.Journal, genetic engineering & biotechnology · 2023Article
- Identification and genetic diversity analysis of hybrid offspring of azalea based on EST-SSR markers.Scientific reports · 2022Article
- Maximizing genetic gain through unlocking genetic variation in different ecotypes of kalmegh (Frontiers in plant science · 2022Article
- De novo transcriptomic analysis and identification of EST-SSR markers in Stephanandra incisa.Scientific reports · 2021Article
- Development and validation of heat-responsive candidate gene and miRNA gene based SSR markers to analysis genetic diversity in wheat for heat tolerance breeding.Molecular biology reports · 2021Article
- Genetic diversity and population structure analysis in a large collection of white clover (PeerJ · 2021Article
- Novel microsatellite markers for evaluation of genetic diversity in the tetraploid flame azalea, Rhododendron calendulaceum (Ericaceae).Molecular biology reports · 2020Article
- Cross-transferability of SSR markers developed in Rhododendron species of Himalaya.Molecular biology reports · 2020Article
- Characterization and Development of Microsatellite Markers inFrontiers in genetics · 2020Article
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7 authors.
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Abstract
The genus Rhododendron, known for large impressive flowers is widely distributed throughout the world. Rhododendrons have limited genetic information, despite of comprising high species diversity, morphological overlap and weak genetic barrier. In present study, expressed sequence tag (EST) data from Rhododendron catawbiense Michx (Subgenus Hymenanthes, Section Ponticum) and Rhododendron mucronatum var. ripense (Makino) E.H. Wilson (Subgenus Tsutsusi, Section Tsutsusi) were utilized for mining and identification of the SSRs for genetic diversity analysis of R. arboreum Smith (Subgenus Tsutsusi, Section Tsutsusi). A total of 249 SSRs were developed from 1767 contigs. Di-nucleotide was found to be most abundant repeat followed by tri- and tetra-nucleotide repeats. The motif AG/CT was most common di-nucleotide motif (31.73%), whereas, AAC/GTT (8.43%), ACG/CGT (8.03%), AAG/CTT (7.23%) and AGG/CCT (6.43%) were most abundant tri-nucleotide repeat motif. Among these SSRs, 168 sequences were only fit into the criteria to design flanking primer pairs. A total of 30 randomly selected primer pairs were utilized for validation and genetic diversity study in 36 genotypes of R. arboreum collected from western Himalayan region. In aggregate, 26 SSR markers (86.66%) produced good and repeatable amplifications. Expected heterozygosity (H
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