ArticleMikrochimica acta2020
Visual detection of microRNA146a by using RNA-functionalized gold nanoparticles.
Article in Mikrochimica acta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Multicomponent nucleic acid enzymes as signal amplification strategy for the detection of microRNA based on fluorescence resonance energy transfer.Mikrochimica acta · 2025Article
- Development of a Gold Nanoparticle-Based Sensor for Authentication of Organic Milk Based on Differential Levels of miRNA.Nanomaterials (Basel, Switzerland) · 2024Article
- Sesame Detection in Food Using DNA-Functionalized Gold Nanoparticles: A Sensitive, Rapid, and Cost-Effective Colorimetric Approach.Biosensors · 2024Article
- Amplification-Free Strategy for miRNA Quantification in Human Serum Using Single Particle ICP-MS and Gold Nanoparticles as Labels.Analytical chemistry · 2024Article
- Nanoplasmonic sensors for extracellular vesicles and bacterial membrane vesicles.Nano convergence · 2024Review
- Advances in Point-of-Care Testing of microRNAs Based on Portable Instruments and Visual Detection.Biosensors · 2023Review
- Using an ultra-compact optical system to improve lateral flow immunoassay results quantitatively.Heliyon · 2022Article
- Determining extracellular vesicles properties and miRNA cargo variability in bovine milk from healthy cows and cows undergoing subclinical mastitis.BMC genomics · 2022Article
- Progress, evolving therapeutic/diagnostic approaches, and challenges in the management of hepatitis C virus infections.Archives of virology · 2022Review
- Emerging roles of noncoding micro RNAs and circular RNAs in bovine mastitis: Regulation, breeding, diagnosis, and therapy.Frontiers in microbiology · 2022Review
- Microchimica Acta topical collection IX NyNA 2019.Mikrochimica acta · 2020Article
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Authors and funding
9 authors.
Funding
Abstract
Gold nanoparticles of different sizes have been synthesized and surface-functionalized with selected RNA probes in order to develop a rapid, low-cost and sensitive method for detection of microRNA146a (miR146a). The strategy is based on the change of colour that can be observed visually after aggregation of the RNA modified-gold nanoparticles (AuNPs) in presence of miR146a. Experimental conditions have been carefully selected in order to obtain a good sensitivity that allows to perform visual detection of microRNA at the nM level, achieving a detection limit of 5 nM. Good repeatability and selectivity versus other sequences that only differ from miR146a in 3 bases was achieved. miR146a has been described as one of the main microRNA involved in the immune response of bovine mastitis, being expressed in tissue, blood and milk samples. The method was successfully applied to the detection of miR146a in raw cow milk samples. The present scheme constitutes a rapid and low-cost alternative to perform highly sensitive detection of microRNA without the need of instrumentation and amplification steps for the early detection of bovine mastitis in the agrofood industry. Graphical abstract Schematic representation of the assay based on aggregation of RNA-modified gold nanoparticles (blue) in presence of microRNA146a generating a dark blue spot onto a solid support, versus a pink spot observed in absence of miR146a due to dispersed gold nanoparticles (red).
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Registered trials
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