Evidence map›Paper›PMID 32123995›Full record

ArticlePlant cell reports2020

Gene expression during development and overexpression after Cercospora kikuchii and salicylic acid challenging indicate defensive roles of the soybean toxin.

Mariana R Arantes, Lucas P Dias, Jose H Costa, Katia D C Saraiva, Janne K S Morais, Daniele O B Sousa, Arlete A Soares, Ilka M Vasconcelos, Jose T A Oliveira

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Article in Plant cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Mariana R ArantesLaboratory of Plant Defense Proteins, Department of Biochemistry and Molecular Biology, Federal University of Ceara, Av. Mister Hull, P.O. Box: 60451, Fortaleza, CE, 60440-900, Brazil.
Lucas P DiasLaboratory of Plant Defense Proteins, Department of Biochemistry and Molecular Biology, Federal University of Ceara, Av. Mister Hull, P.O. Box: 60451, Fortaleza, CE, 60440-900, Brazil. lpinheirodias@gmail.com.
Jose H CostaLaboratory of Plant Defense Proteins, Department of Biochemistry and Molecular Biology, Federal University of Ceara, Av. Mister Hull, P.O. Box: 60451, Fortaleza, CE, 60440-900, Brazil.
Katia D C SaraivaLaboratory of Plant Defense Proteins, Department of Biochemistry and Molecular Biology, Federal University of Ceara, Av. Mister Hull, P.O. Box: 60451, Fortaleza, CE, 60440-900, Brazil.
Janne K S MoraisLaboratory of Plant Defense Proteins, Department of Biochemistry and Molecular Biology, Federal University of Ceara, Av. Mister Hull, P.O. Box: 60451, Fortaleza, CE, 60440-900, Brazil.
Daniele O B SousaLaboratory of Plant Defense Proteins, Department of Biochemistry and Molecular Biology, Federal University of Ceara, Av. Mister Hull, P.O. Box: 60451, Fortaleza, CE, 60440-900, Brazil.
Arlete A SoaresDepartment of Biology, Federal University of Ceara, Fortaleza, CE, 60440-900, Brazil.
Ilka M VasconcelosLaboratory of Plant Defense Proteins, Department of Biochemistry and Molecular Biology, Federal University of Ceara, Av. Mister Hull, P.O. Box: 60451, Fortaleza, CE, 60440-900, Brazil.
Jose T A OliveiraLaboratory of Plant Defense Proteins, Department of Biochemistry and Molecular Biology, Federal University of Ceara, Av. Mister Hull, P.O. Box: 60451, Fortaleza, CE, 60440-900, Brazil. jtaolive@ufc.br.ORCID http://orcid.org/0000-0003-1207-1140

Funding

CNPq 140780/2015-7CNPq Grant 307783/2013-8CNPq Grant 308107/2013-6CNPq Grant 310694/2014-0CNPq Grant 431511/2016-0
6 · The paper itself

Abstract

key messageSBTX has defensive role against C. kikuchii, and therefore, its constituent genes SBTX17 and SBTX27 are promising candidates to engineer pathogen resistant plants. Soybean (Glycine max [L.] Merr.) is economically the most important legume crop in the world. Its productivity is strongly affected by fungal diseases, which reduce soybean production and seed quality and cause losses of billions of dollars worldwide. SBTX is a protein that apparently takes part in the defensive chemical arsenal of soybean against pathogens. This current study provides data that reinforce this hypothesis. Indeed, SBTX inhibited in vitro the mycelial growth of Cercospora kikuchii, it is constitutively located in the epidermal region of the soybean seed cotyledons, and it is exuded from mature imbibed seeds. Moreover, RT-qPCR analysis of the SBTX associated genes, SBTX17 and SBTX27, which encode for the 17 and 27 kDa polypeptide chains, showed that both genes are expressed in all studied plant tissues during the soybean development, with the highest levels found in the mature seeds and unifoliate leaves. In addition, to assess a local response of the soybean secondary leaves from 35-day-old plants, they were inoculated with C. kikuchii and treated with salicylic acid. It was verified using RT-qPCR that SBTX17 and SBTX27 genes overexpressed in leaves compared to controls. These findings strongly suggest that SBTX has defensive roles against C. kikuchii. Therefore, SBTX17 and SBTX27 genes are promising candidates to engineer pathogen resistant plants.

Indexed as

AscomycotaCotyledonDisease ResistanceGene ExpressionGene Expression Regulation, PlantGlycine maxGlycoproteinsPlant DiseasesPlant LeavesPromoter Regions, GeneticSalicylic AcidSeedsSoybean ProteinsUp-RegulationGlycoproteinsSalicylic AcidSBTX protein, soybeanSoybean ProteinsCercospora kikuchiiDefense proteinGene expressionGlycine maxSBTX

Identifiers

PMID32123995

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.