ArticleProceedings of the National Academy of Sciences of the United States of America2020
Charge-dependent interactions of monomeric and filamentous actin with lipid bilayers.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
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- Ionic Regulation of Mechanosurveillance and Metastasis via the MRTFA/KCNMB1 Axis.bioRxiv : the preprint server for biology · 2026Article
- Peptide Electrostatic Modulation Directs Human Neural Cell Fate.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Histidine 73 methylation coordinates β-actin plasticity in response to key environmental factors.Nature communications · 2025Article
- Lipid Membrane Domains Control Actin Network Viscoelasticity.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Benefits and challenges of reconstituting the actin cortex.Cytoskeleton (Hoboken, N.J.) · 2024Review
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- The molecular picture of the local environment in a stable model coacervate.Communications chemistry · 2024Article
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- Understanding and fine tuning the propensity of ATP-driven liquid-liquid phase separation with oligolysine.Physical chemistry chemical physics : PCCP · 2024Article
- Unbreaking Assemblies in Molecular Simulations with Periodic Boundaries.Journal of chemical information and modeling · 2023Article
- Self-organizing actin networks drive sequential endocytic protein recruitment and vesicle release on synthetic lipid bilayers.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Bioinspired Membrane Interfaces: Controlling Actomyosin Architecture and Contractility.ACS applied materials & interfaces · 2023Article
- Self-organizing actin networks drive sequential endocytic protein recruitment and vesicle release on synthetic lipid bilayers.bioRxiv : the preprint server for biology · 2023Article
- Capturing coacervate formation and protein partition by molecular dynamics simulation.Chemical science · 2023Article
- Human and animal exposure to newly discovered sand fly viruses, China.Frontiers in cellular and infection microbiology · 2023Article
- Actomyosin-Driven Division of a Synthetic Cell.ACS synthetic biology · 2022Review
- Molecular mechanism of CD44 homodimerization modulated by palmitoylation and membrane environments.Biophysical journal · 2022Article
- Identification of electroporation sites in the complex lipid organization of the plasma membrane.eLife · 2022Article
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cytoskeletal protein actin polymerizes into filaments that are essential for the mechanical stability of mammalian cells. In vitro experiments showed that direct interactions between actin filaments and lipid bilayers are possible and that the net charge of the bilayer as well as the presence of divalent ions in the buffer play an important role. In vivo, colocalization of actin filaments and divalent ions are suppressed, and cells rely on linker proteins to connect the plasma membrane to the actin network. Little is known, however, about why this is the case and what microscopic interactions are important. A deeper understanding is highly beneficial, first, to obtain understanding in the biological design of cells and, second, as a possible basis for the building of artificial cortices for the stabilization of synthetic cells. Here, we report the results of coarse-grained molecular dynamics simulations of monomeric and filamentous actin in the vicinity of differently charged lipid bilayers. We observe that charges on the lipid head groups strongly determine the ability of actin to adsorb to the bilayer. The inclusion of divalent ions leads to a reversal of the binding affinity. Our in silico results are validated experimentally by reconstitution assays with actin on lipid bilayer membranes and provide a molecular-level understanding of the actin-membrane interaction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.