ArticleProceedings of the National Academy of Sciences of the United States of America2020
Mechanophenotyping of 3D multicellular clusters using displacement arrays of rendered tractions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Label-free all-electrical tracking of individual and collective cell migration on a megapixel CMOS capacitance sensor.Lab on a chip · 2026Article
- Label-Free All-Electrical Tracking of Individual and Collective Cell Migration on a Megapixel CMOS Capacitance Sensor.bioRxiv : the preprint server for biology · 2026Article
- Partial EMT Drives Persistent Collective Migration via Collision Guidance in Heterogeneous Populations.bioRxiv : the preprint server for biology · 2026Article
- Guidance for 3D traction force microscopy today and in the next decade.Nature methods · 2026Review
- Mapping Collective Forces of Lung Cancer Spheroids Using Traction Force Microscopy.Biotechnology and bioengineering · 2026Article
- Expression of vimentin intermediate filaments in epithelial cells promotes cell migration and cell matrix interaction in 3D.bioRxiv : the preprint server for biology · 2025Article
- Vimentin intermediate filaments as structural and mechanical coordinators of mesenchymal cells.Nature cell biology · 2025Review
- Confined cell migration along extracellular matrix space in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- 3D Traction Force Microscopy in Biological Gels: From Single Cells to Multicellular Spheroids.Annual review of biomedical engineering · 2024Review
- Laser speckle rheological microscopy reveals wideband viscoelastic spectra of biological tissues.Science advances · 2024Article
- Advances in high throughput cell culture technologies for therapeutic screening and biological discovery applications.Bioengineering & translational medicine · 2024Review
- Generative modeling of biological shapes and images using a probabilisticbioRxiv : the preprint server for biology · 2024Article
- Local response and emerging nonlinear elastic length scale in biopolymer matrices.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Mechanobiology in oncology: basic concepts and clinical prospects.Frontiers in cell and developmental biology · 2023Review
- Recent advances in optical elastography and emerging opportunities in the basic sciences and translational medicine [Invited].Biomedical optics express · 2023Review
- Reciprocity of Cell Mechanics with Extracellular Stimuli: Emerging Opportunities for Translational Medicine.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- Light-sheet photonic force optical coherence elastography for high-throughput quantitative 3D micromechanical imaging.Nature communications · 2022Article
- Generation, Transmission, and Regulation of Mechanical Forces in Embryonic Morphogenesis.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- Integrating mechanical sensor readouts into organ-on-a-chip platforms.Frontiers in bioengineering and biotechnology · 2022Review
- Revisiting tissue tensegrity: Biomaterial-based approaches to measure forces across length scales.APL bioengineering · 2021Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Epithelial tissues mechanically deform the surrounding extracellular matrix during embryonic development, wound repair, and tumor invasion. Ex vivo measurements of such multicellular tractions within three-dimensional (3D) biomaterials could elucidate collective dissemination during disease progression and enable preclinical testing of targeted antimigration therapies. However, past 3D traction measurements have been low throughput due to the challenges of imaging and analyzing information-rich 3D material deformations. Here, we demonstrate a method to profile multicellular clusters in a 96-well-plate format based on spatially heterogeneous contractile, protrusive, and circumferential tractions. As a case study, we profile multicellular clusters across varying states of the epithelial-mesenchymal transition, revealing a successive loss of protrusive and circumferential tractions, as well as the formation of localized contractile tractions with elongated cluster morphologies. These cluster phenotypes were biochemically perturbed by using drugs, biasing toward traction signatures of different epithelial or mesenchymal states. This higher-throughput analysis is promising to systematically interrogate and perturb aberrant mechanobiology, which could be utilized with human-patient samples to guide personalized therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.